Mutation rate and novel mutants of induced by carbon ions

Shikazono, Naoya; Yokota, Yukihiko*; Kitamura, Satoshi; Suzuki, Chihiro*; Watanabe, Hiroshi; Tano, Shigemitsu; Tanaka, Atsushi

Genetics, 163(4), p.1449 - 1455, 2003/04

https://doi.org/10.1093/genetics/163.4.1449

Irradiation of by carbon ions was carried out to investigate the mutational effect of ion particles in higher plants. Frequencies of embryonic lethals and chlorophyll deficient mutants were found to be significantly higher after carbon ion-irradiation than after electron-irradiation (11-fold and 7.8-fold per unit dose, respectively). During the isolation of mutants, two new classes of flavonoid mutants () were isolated from carbon ion-mutagenized M2 plants. From PCR and sequence analysis, two of the three mutant alleles were found to have a small deletion within the gene and the other was revealed to contain a rearrangement. Using the segregation rates, the mutation rate of carbon ions was estimated to be 17-fold higher than that of electrons. The isolation of novel mutants and the high mutation rate suggest that ion particles can be used as a valuable mutagen for plant genetics.

LET dependence of lethality in irradiated by heavy ions

Shikazono, Naoya; Tanaka, Atsushi; Kitayama, Shigeru*; Watanabe, Hiroshi; Tano, Shigemitsu*

Radiation and Environmental Biophysics, 41(2), p.159 - 162, 2002/04

https://doi.org/10.1007/s00411-002-0157-4

no abstracts in English

Proceedings of the 8th Workshop on Plant Mutation Breeding; Effective use of Physical/Chemical Mutagens, October 9-13, 2000, Hanoi, Vietnam

Kume, Tamikazu; Watanabe, Kazuo*; Tano, Shigemitsu

JAERI-Conf 2001-003, 209 Pages, 2001/03

JAERI-Conf-2001-003.pdf:15.4MB

no abstracts in English

Nucleotide sequence of the GLABROUSI gene of arabidopsis thaliana ecotype columbia

Shikazono, Naoya; ; Yokota, Yukihiko*; Watanabe, Hiroshi; Tano, Shigemitsu*

DNA Sequence, 9(3), p.177 - 181, 1998/00

no abstracts in English

The Genomic organization of the arabidopsis 6-4 photolyase gene

Sakamoto, Ayako; ; Tano, Shigemitsu*; *; Yamamoto, Kazuo*; Watanabe, Hiroshi

Plant Physiol. (Plant Gene Register PGR98-180), 118, 1101 Pages, 1998/00

https://doi.org/10.1104/pp.118.3.1101

no abstracts in English

A New mutant induced by ion beams affects flavonoid synthesis with spotted pigmentation in testa

Tanaka, Atsushi; Tano, Shigemitsu*; Chantes, T.*; Yokota, Yukihiko*; Shikazono, Naoya; Watanabe, Hiroshi

Genes and Genetic Systems, 72(3), p.141 - 148, 1997/00

https://doi.org/10.1266/ggs.72.141

no abstracts in English

Penetration controlled irradiation with ion beams for biological study

; Watanabe, Hiroshi; Shimizu, Takashi*; Inoue, Masayoshi*; Kikuchi, Masahiro; Kobayashi, Yasuhiko; Tano, Shigemitsu*

Nuclear Instruments and Methods in Physics Research B, 129(1), p.42 - 48, 1997/00

https://doi.org/10.1016/S0168-583X(97)00131-6

no abstracts in English

Biological effects of heavy charged particles in Arabidopsis thaliana

; Yokota, Yukihiko*; Watanabe, Hiroshi; Shikazono, Naoya; Tano, Shigemitsu*

GSI-95-10, 0, p.87 - 90, 1995/00

no abstracts in English

Reduced rejoining ability of DNA strand breaks with differentiation in barley root cells

Shikazono, Naoya; Watanabe, Hiroshi; ; Tano, Shigemitsu*; *; *

Mutat. Res., DNA Repair, 337, p.41 - 48, 1995/00

no abstracts in English

Biological effects of ion beams and its application

Watanabe, Hiroshi; Tano, Shigemitsu*

Genshiryoku Kogyo, 40(2), p.45 - 49, 1994/00

http://ci.nii.ac.jp/naid/40001074295

no abstracts in English