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Kitamura, Satoshi; Matsuda, Fumio*; Toge, Takayuki*; Sakakibara, Keiko*; Yamazaki, Mami*; Saito, Kazuki*; Narumi, Issei
JAEA-Review 2010-065, JAEA Takasaki Annual Report 2009, P. 60, 2011/01
Kitamura, Satoshi; Matsuda, Fumio*; Toge, Takayuki*; Sakakibara, Keiko*; Yamazaki, Mami*; Saito, Kazuki*; Narumi, Issei
Plant Journal, 62(4), p.549 - 559, 2010/05
Times Cited Count：81 Percentile：89.47(Plant Sciences)Arabidopsis TT19 encodes a GST-like protein that is involved in the accumulation of proanthocyanidins (PAs) in the seed coat. PA accumulation sites in tt19 immature seeds were observed as small vacuolar-like structures, which show unique thick morphology by differential interference contrast microscopy. The distribution pattern of the thick structures overlapped the location of PA accumulation sites in tt19. Metabolic profiling of the solvent-soluble fraction demonstrated that PA derivatives such as epicatechins and epicatechin oligomers, although highly accumulated in the wild-type, were absent in tt19. We also revealed that tt12 specifically accumulated glycosylated epicatechins, the putative transport substrates for TT12. Given the cytosolic localization of functional GFP-TT19 proteins, our results suggest that TT19, which acts prior to TT12, functions in cytosol for regular accumulation of PA precursors such as epicatechin and glycosylated epicatechin in the vacuole.
Kitamura, Satoshi; Toge, Takayuki*; Matsuda, Fumio*; Sakakibara, Keiko*; Saito, Kazuki*; Narumi, Issei
no journal, ,
Flavonoids are widely distributed in the plant kingdom, and their functions as antimicrobial agents and UV-B protectants are essential for the normal growth of plants under stressful environments. Flavonoid biosynthesis is thought to proceed in the cytosolic surface of the endoplasmic reticulum, whereas many endproducts of flavonoid are accumulated in vacuoles. In the model plant Arabidopsis, intracellular flavonoid transport mechanisms are investigated for proanthocyanidin pathway, one of the flavonoid subclass being synthesized and accumulated specifically in seed coat. Some factors presumably involved in proanthocyanidin transport/accumulation, such as membrane-bound proanthocyanidin transporter TT12 and putative proanthocyanidin ligandin TT19, have so far been isolated. We describe here the results on metabolic analysis of immature seed extracts from these flavonoid mutants.
Kitamura, Satoshi; Matsuda, Fumio*; Toge, Takayuki*; Sakakibara, Keiko*; Yamazaki, Mami*; Saito, Kazuki*; Narumi, Issei
no journal, ,
Arabidopsis TRANSPARENT TESTA19 (TT19) is involved in the accumulation of proanthocyanidins (PAs) in the seed coat. PA accumulation sites in tt19 immature seeds were observed as small vacuolar-like structures, whereas those in tt12, a mutant of the tonoplast-bound transporter of PAs, and tt12 tt19 were observed at peripheral regions of small vacuoles. We found that tt19 immature seeds had small spherical structures showing unique thick morphology by differential interference contrast microscopy. The thick structures were outlined with GFP-TT12 proteins in tt19. Metabolic profiling demonstrated that PA derivatives such as epicatechins and epicatechin oligomers were absent in tt19. Given the cytosolic localization of functional GFP-TT19 proteins, the PA pathway in Arabidopsis seed coat is discussed in relation to the subcellular localization of PA metabolites.
Kitamura, Satoshi; Narumi, Issei; Toge, Takayuki*; Yamazaki, Mami*; Saito, Kazuki*; Matsuda, Fumio*; Sakakibara, Keiko*
no journal, ,
no abstracts in English
