Involvement of Connexin43 hemichannel in ATP release after -irradiation

Ohshima, Yasuhiro; Tsukimoto, Mitsutoshi*; Harada, Hitoshi*; Kojima, Shuji*

Journal of Radiation Research, 53(4), p.551 - 557, 2012/07

https://doi.org/10.1093/jrr/rrs014

Immune response pathways in human keratinocyte (HaCaT) cells are induced by ultraviolet B via p38 mitogen-activated protein kinase activation

Muto, Yasuko; Tsukimoto, Mitsutoshi*; Homma, Takujiro*; Kojima, Shuji*

Journal of Health Science, 56(6), p.675 - 683, 2010/00

https://doi.org/10.1248/jhs.56.675

Biological assessment of damaged ATP by ionizing radiations

Fujii, Kentaro; Fujii, Shinichiro*; Akimitsu, Nobuyoshi*; Tsukimoto, Mitsutoshi*; Kojima, Shuji*

no journal, , 

We analyze the luciferase activity to assess the energy transfer activity and the genetic information transfer activity and evaluate the change in activity as an inter-cellular signaling molecules by measuring ATP receptor-mediated phosphorylation of extra cellular signal-regulated kinase 1/2 (ERK1/2). In addition to the biological effect, we have also measured the molecular alterations using X-ray absorption and electro spray mass spectrometry (ESIMS). We observed attenuation of ATP receptor-mediated phosphorylation of ERK 1/2 and luciferase activity by the soft X-ray irradiations. Moreover, we observed the reduction of nucleotide from the X-ray absorption spectra and ESIMS. These results suggest that certain alteration of ATP structure induced by soft X-ray affects the biological function of ATP.

X-ray absorption near edge structure of ATP thin film irradiated with soft X-rays

Fujii, Kentaro; Fujii, Shinichiro*; Kato, Dai*; Tsukimoto, Mitsutoshi*; Akimitsu, Nobuyoshi*; Narita, Ayumi; Nakajima, Takafumi*; Ide, Shunta*; Salam, K. A.*; Niwa, Osamu*; et al.

no journal, ,