Mutation analysis of high ethyl caproate producing sake yeasts generated by ion beam breeding

Masubuchi, Takashi*; Ueda, Ryoshiro; Kamiyama, Osamu*; Ikenaga, Hiroshi*; Sato, Katsuya; Narumi, Issey*

JAEA-Review 2013-059, JAEA Takasaki Annual Report 2012, P. 117, 2014/03

Decolorization of secondary treated water from livestock urine waste

Takigami, Machiko*; Nagasawa, Naotsugu; Hiroki, Akihiro; Kasai, Noboru; Yoshii, Fumio; Tamada, Masao; Takigami, Shoji*; Shibata, Takuya*; Aketagawa, Yasushi*; Ozaki, Masuo*

Transactions of the Materials Research Society of Japan, 35(3), p.647 - 650, 2010/09

https://doi.org/10.14723/tmrsj.35.647

There are many livestock farmers in Gunma Prefecture. Many of the farms are located under Mt. Akagi and Haruna, which attract tourists. Furthermore, rivers around the mountains are source of water supply to metropolitan area. Therefore, the waste treatments are very important. Regulations of smell and COD (chemical oxygen demand) are severe in Gunma Prefecture. Although there is no regulation for color of drain water, color in secondary treated water from livestock urine waste has been one of the major concerns for livestock farmers. The color is a metabolite of microorganisms and not easy to be removed. People have tried to remove the color by ozone treatment, absorption using activated carbon or soils. An absorbent was prepared by radiation grafting; fibers were irradiated with Co -rays and some kinds of monomers were grafted onto the fibers. The absorbent thus prepared were used to decolorize the secondary treated water. The color and COD were removed by the absorbent, however, COD removal was more difficult than decolorization. Degree of grafting (weight increase expressed in % by grafting reaction to initial weight of the fiber), treated water/absorbent ratio, flow rate of treated water, and direction of flow affected color and COD removal. The used absorbent could be used repeatedly after washing.

Gene expression analysis of sake yeast generated by ion beam breeding technology

Tomizawa, Yuki*; Hayashi, Hidenori*; Masubuchi, Takashi*; Kamiyama, Osamu*; Sato, Katsuya; Narumi, Issei; Ikenaga, Hiroshi*

no journal, , 

no abstracts in English

Mutation analysis of high ethyl caproate producing sake yeasts generated by ion beam breeding

Sato, Katsuya; Ueda, Ryoshiro; Masubuchi, Takashi*; Kamiyama, Osamu*; Hayashi, Hidenori*; Ikenaga, Hiroshi*; Narumi, Issey*; Tanaka, Atsushi

no journal, , 

We have previously obtained high ethyl caproate producing Sake yeasts "No.227" and "No.1333" by ion beam breeding. The fatty acid synthase genes ( and ) and the fatty acid esterase genes ( and ) might be involved in the higher production of ethyl caproate in the mutant strains. In this study, to identify the mutation sites of the mutant strains, each gene locus was sequenced. For the , and loci, all the mutations found in mutant strains are nonsense mutations, suggesting that these mutations did not involved in the higher production of ethyl caproate. On the other hand, strains No.227 and No.1333 carried G/A and A/A mutations in gene at nucleotide position 3,748 as heterozygous and homozygous states, respectively. This result strongly suggested that the higher production of ethyl caproate in strains No.227 and No.1333 is attributed to a dominant mutation in gene.

Mutation analysis of high ethyl caproate producing sake yeasts generated by ion beam breeding

Masubuchi, Takashi*; Kamiyama, Osamu*; Ueda, Ryoshiro; Ikenaga, Hiroshi*; Hayashi, Hidenori*; Sato, Katsuya; Narumi, Issey*

no journal, , 

We have previously obtained high ethyl caproate producing Sake yeasts "No.227" and "No.1333" by ion beam breeding. The fatty acid synthase genes ( and ) and the fatty acid esterase genes ( and ) might be involved in the higher production of ethyl caproate in the mutant strains. In this study, to identify the mutation sites of the mutant strains, each gene locus was sequenced. For the , and loci, all the mutations found in mutant strains are nonsense mutations, suggesting that these mutations did not involved in the higher production of ethyl caproate. On the other hand, strains No.227 and No.1333 carried G/A and A/A mutations in gene at nucleotide position 3,748 as heterozygous and homozygous states, respectively. This result strongly suggested that the higher production of ethyl caproate in strains No.227 and No.1333 is attributed to a dominant mutation in gene.

Analysis of sake yeast transcription system of galactose assimilating genes

Ikenaga, Hiroshi*; Tomizawa, Yuki*; Hikokubo, Kazuya*; Hayashi, Hidenori*; Masubuchi, Takashi*; Kamiyama, Osamu*; Sato, Katsuya; Narumi, Issey*

no journal, , 

no abstracts in English