Analysis of bystander effect induced by cell membrane response in glioma cells

Wada, Seiichi*; Ando, Tatsuhiko*; Watanabe, Aya*; Kakizaki, Takehiko*; Natsuhori, Masahiro*; Funayama, Tomoo; Sakashita, Tetsuya; Yokota, Yuichiro; Kobayashi, Yasuhiko

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 79, 2015/03

https://doi.org/10.11484/jaea-review-2014-050

So far, we clarified that X-ray irradiation induced cell killing by bystander effect mediated-secreted factor. This phenomenon was related with sphingomyelinase (SMase). In this study we analyzed mechanism of secreted SMase from irradiated cells after irradiation. SMase was detected in the culture medium after irradiation by SDS-PAGE. Then, SMase was detected in the exosome of culture medium, but not out of exosome after irradiation. This result indicates that SMase was secreted as exosome from the irradiated cells.

The Characteristics of radiation-induced cell responses in the canine spontaneous tumor cells

Sano, Tadashi*; Wada, Seiichi*; Suzuki, Keiko*; Natsuhori, Masahiro*; Kakizaki, Takehiko; Ito, Takashi*; Nakazawa, Konomi*; Kobayashi, Yasuhiko; Ito, Nobuhiko*

JAEA-Review 2006-042, JAEA Takasaki Annual Report 2005, P. 115, 2007/02

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2006-042.pdf

Distinct modes of cell death by ionizing radiation observed in two lines of feline T-lymphocytes

Kakizaki, Takehiko; Hamada, Nobuyuki*; Wada, Seiichi*; Funayama, Tomoo; Sakashita, Tetsuya; Hodatsu, Tsutomu*; Sano, Tadashi*; Natsuhori, Masahiro*; Kobayashi, Yasuhiko; Ito, Nobuhiko*

Journal of Radiation Research, 47(3-4), p.237 - 243, 2006/11

no abstracts in English

Detection of DNA damage induced by heavy ion irradiation in the individual cells with comet assay

Wada, Seiichi; Natsuhori, Masahiro*; Ito, Nobuhiko*; Funayama, Tomoo; Kobayashi, Yasuhiko

Nuclear Instruments and Methods in Physics Research B, 206, p.553 - 556, 2003/05

https://doi.org/10.1016/S0168-583X(03)00820-6

Determining the biological effects of a very low number of charged particles crossing the cell nucleus is interest for estimating the risk due to environmental exposure to charged particles. Especially it is necessary to detect the radiation damage induced by a precise number of charged particles in the individual cells. To compare the number of ions traversing the cell and the DNA damage produced by the hit ions, we applied comet assay. Cells attached on the ion track detector CR-39 were irradiated with 17.3 MeV/u 12C, 15.7 MeV/u, 10.4 MeV/u 20Ne and 6.9 MeV/u 40Ar ion beams at TIARA, JAERI-Takasaki. After irradiation, CR-39 was covered with 1 % agarose. After electrophoresis the CR-39 was taken off from the slide glass. The agarose gel on the CR-39 was stained with ethidium bromide and the opposite side of the CR-39 was etched with KOH-ethanol solution at 37 . We observed that the ion particles with higher LET value induced the heavier DNA damage, even by the same number of ion-hits within the irradiated cells.

Cell killing effects of charged particles on feline T-lymphocytes

Kakizaki, Takehiko; Hamada, Nobuyuki*; Wada, Seiichi*; Sakashita, Tetsuya; Hara, Takamitsu*; Hodatsu, Tsutomu*; Natsuhori, Masahiro*; Sano, Tadashi*; Funayama, Tomoo; Fukamoto, Kana; et al.

no journal, , 

no abstracts in English

Cell killing effects of charged particles on feline T-lymphocytes

Kakizaki, Takehiko; Hamada, Nobuyuki*; Wada, Seiichi*; Sakashita, Tetsuya; Funayama, Tomoo; Hodatsu, Tsutomu*; Natsuhori, Masahiro*; Sano, Tadashi*; Kobayashi, Yasuhiko; Ito, Nobuhiko*

no journal, , 

no abstracts in English

Relationship between ion track structure and quality of cellular DNA damage

Wada, Seiichi*; Funayama, Tomoo; Sakashita, Tetsuya; Hamada, Nobuyuki*; Kakizaki, Takehiko*; Ito, Nobuhiko*; Kobayashi, Yasuhiko

no journal, , 

CHO-K1 cells attached on ion track detector CR-39 were irradiated with the same LET value but different nuclide (C and Ne). DNA strand breaks were immunohistochemically detected with tdt for total breaks, and with H2AX antibody for double strand breaks only. The fluorescent signal observed in nuclei by immunochemistry was co-localized at the site of etched pit where the ion was traversed on the cell. To evaluate the spatial distribution of DNA damage, the diameter of the area of fluorescent signal was measured. The area of H2AX induced by C ion and Ne ion were not significantly different, however, the area of DNA strand breaks induced by Ne ion was larger than that induced by C ion. This result indicates that the difference of ion track structure influences the pattern of induction of DNA damage.