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Journal Articles

Target mutation breeding of flower color by taking advantage of ion-beam irradiation and genomic information

Tanaka, Atsushi

Plant Biotechnology, 29(3), p.191 - 192, 2012/06

 Times Cited Count:4 Percentile:72.85(Biotechnology & Applied Microbiology)

We launched a project to overcome the randomness of mutation and to develop logical and efficient mutation breeding by taking advantage of both ion-beam irradiation and genomic information. This research was supported by a five-year grant from the Research and Development Program for New Bio-industry Initiatives of the Bio-oriented Technology Research Advancement Institution (BRAIN). To achieve our purpose, we focused on flower-color mutations, because flavonoid biosynthesis is one of the most well-known molecular mechanisms in higher plants. We envisioned four ideal target plants for mutation breeding. These were glittering carnation, red creeping petunia, and blue-purple and crimson fragrant cyclamen. Several new factors that strongly affect flower color were identified from this project: (1) Non-acylation was found in a glittering carnation petal. (2) A loss-of-function mutation in GST (glutathione S-transferase) caused not only a decrease of pigmentation but also a change in flower color in carnation. (3) Carnation flower color was strongly affected by the function of AA5GT (acyl-glucose dependent anthocyanin 5-glucosyltransferase). (4) Delphinidins caused cyclamen flowers to turn red-purple. (5) A decrease in flavonol metabolism caused increased amounts of pigmentation in cyclamen flowers.

Journal Articles

Isolation and characterization of the fragrant cyclamen ${it O}$-methyltransferase involved in flower coloration

Akita, Yusuke; Kitamura, Satoshi; Hase, Yoshihiro; Narumi, Issei; Ishizaka, Hiroshi*; Kondo, Emiko*; Kameari, Naoko*; Nakayama, Masayoshi*; Tanikawa, Natsu*; Morita, Yasumasa*; et al.

Planta, 234(6), p.1127 - 1136, 2011/12

 Times Cited Count:35 Percentile:75.42(Plant Sciences)

Journal Articles

Efficient induction of flower-color mutants by ion beam irradiation in petunia seedlings treated with high sucrose concentration

Hase, Yoshihiro; Okamura, Masachika*; Takeshita, Daigaku*; Narumi, Issei; Tanaka, Atsushi

Plant Biotechnology, 27(1), p.99 - 103, 2010/03

 Times Cited Count:25 Percentile:60.15(Biotechnology & Applied Microbiology)

Journal Articles

Red-purple flower due to delphinidin 3,5-diglucosides, a novel pigment for ${it Cyclamen}$ spp., generated by ion-beam irradiation

Kondo, Emiko*; Nakayama, Masayoshi*; Kameari, Naoko*; Tanikawa, Natsu*; Morita, Yasumasa*; Akita, Yusuke; Hase, Yoshihiro; Tanaka, Atsushi; Ishizaka, Hiroshi*

Plant Biotechnology, 26(5), p.565 - 569, 2009/01

 Times Cited Count:33 Percentile:66.96(Biotechnology & Applied Microbiology)

Fragrant cyclamen cultivar (${it C. persicum}$ $$times$$ ${it C. purpurascens}$), that bloomed purple flower containing malvidin 3,5-diglucoside as the major anthocyanin, was irradiated with a 320-MeV carbon-ion beam at 0-16 Gy to increase flower color variation by mutation. Some of the M2 plants derived from self-pollination of M1 plants irradiated at 2 Gy were flower-color mutants that retained desirable flower shape, flower size, and leaf color. One of the mutants bloomed novel red-purple flowers, the major anthocyanin of which was delphinidin 3,5-diglucoside. Because the major anthocyanins in flowers of ${it Cyclamen}$ spp. were previously restricted to malvidin, peonidin, and cyanidin types, the generation of a cyclamen containing mostly the delphinidin-type anthocyanin is an important breakthrough in cyclamen breeding. We expect this mutant to become not only a commercial cultivar itself, but also a valuable genetic resource for cyclamen breeding.

Oral presentation

Comparative analysis of floral pigmentation between Cyclamen graecum and its colorless mutant

Akita, Yusuke; Ishizaka, Hiroshi*; Shimada, Akihiko; Nakayama, Masayoshi*; Kitamura, Satoshi; Hase, Yoshihiro; Tanaka, Atsushi; Narumi, Issei

no journal, , 

no abstracts in English

Oral presentation

Mutation induction in fragrance cyclamen (${it Cyclamen persicum}$ x ${it C. purpurascens}$) by ion beam re-irradiation

Kondo, Emiko*; Kameari, Naoko*; Nakayama, Masayoshi*; Akita, Yusuke; Hase, Yoshihiro; Tanikawa, Natsu*; Morita, Yasumasa*; Ishizaka, Hiroshi*

no journal, , 

no abstracts in English

Oral presentation

Comparative analysis of flavonoid biosynthesis genes between ${it Cyclamen graecum}$ and its white-flowered mutant

Akita, Yusuke; Ishizaka, Hiroshi*; Nakayama, Masayoshi*; Shimada, Akihiko; Kitamura, Satoshi; Hase, Yoshihiro; Tanaka, Atsushi; Narumi, Issei

no journal, , 

Oral presentation

Efficient induction of flower-color mutants by ion beam irradiation in petunia seedlings treated with high sucrose concentration

Hase, Yoshihiro; Narumi, Issei; Tanaka, Atsushi; Okamura, Masachika*; Takeshita, Daigaku*

no journal, , 

no abstracts in English

Oral presentation

Induction of flower color mutation in fragrance cyclamen, "Kaori-no-mai" by ion beam irradiation

Kondo, Emiko*; Nakayama, Masayoshi*; Kameari, Naoko*; Tanikawa, Natsu*; Morita, Yasumasa*; Akita, Yusuke; Hase, Yoshihiro; Tanaka, Atsushi; Ishizaka, Hiroshi*

no journal, , 

One of the fragrant cyclamens, Kaori-no-mai, blooms purple flowers containing malvidin 3,5-diglucoside as the major anthocyanin. Here, we irradiated etiolated petioles of Kaori-no-mai with a 320-MeV carbon-ion beam to increase flower color variation by mutation. Some of the M2 plants derived from self-pollination of M1 plants were flower-color mutan. One of the mutants bloomed novel red-purple flowers, the major anthocyanin of which was delphinidin 3,5-diglucoside. The major anthocyanins in flowers of ${it Cyclamen }$ spp. were previously restricted to malvidin, peonidin, and cyanidin types, therefore, the generation of a cyclamen containing mostly the delphinidin-type anthocyanin is an important breakthrough in cyclamen breeding. We expect this mutant to become not only a commercial cultivar itself, but also a valuable genetic resource for cyclamen breeding.

Oral presentation

Efficient induction of flower color mutants by ion beam irradiation in petunia seedlings treated with high sucrose concentration

Hase, Yoshihiro; Okamura, Masachika*; Takeshita, Daigaku*; Narumi, Issei; Tanaka, Atsushi

no journal, , 

no abstracts in English

Oral presentation

Mutation induction in fragrance cyclamen "Uruwashi-no-kaori" by ion beam irradiation

Kondo, Emiko*; Kameari, Naoko*; Nakayama, Masayoshi*; Kurihara, Yasushi*; Akita, Yusuke; Tanikawa, Natsu*; Morita, Yasumasa*; Hase, Yoshihiro; Tanaka, Atsushi; Ishizaka, Hiroshi*

no journal, , 

no abstracts in English

Oral presentation

Analyses of flower pigments and volatile compounds of red-purple mutant generated by ion beam irradiation from haploid of fragrant purple cyclamen "Kaori-no-mai"(${it Cyclamen persicum}$ $$times$$ ${it C. purpurascens}$)

Kameari, Naoko*; Nakayama, Masayoshi*; Kondo, Emiko*; Kurihara, Yasushi*; Akita, Yusuke; Hase, Yoshihiro; Tanikawa, Natsu*; Morita, Yasumasa*; Tanaka, Atsushi; Ishizaka, Hiroshi*

no journal, , 

no abstracts in English

Oral presentation

Analyses of flower pigments and volatile compounds of red-purple mutants generated by ion beam irradiation from fragrant purple cyclamen "Kaori-no-mai"(${it Cyclamen persicum}$ $$times$$ ${it C. purpurascens}$)

Kondo, Emiko*; Nakayama, Masayoshi*; Kameari, Naoko*; Kurihara, Yasushi*; Tanikawa, Natsu*; Morita, Yasumasa*; Akita, Yusuke; Hase, Yoshihiro; Tanaka, Atsushi; Ishizaka, Hiroshi*

no journal, , 

no abstracts in English

Oral presentation

Analyses of flower pigments and volatile compounds of white mutants generated by ion beam irradiation from fragrant purple cyclamen "Kokou-no-kaori"(${it Cyclamen persicum}$ $$times$$ ${it C. purpurascens}$)

Kondo, Emiko*; Nakayama, Masayoshi*; Kameari, Naoko*; Kurihara, Yasushi*; Tanikawa, Natsu*; Morita, Yasumasa*; Akita, Yusuke; Hase, Yoshihiro; Tanaka, Atsushi; Ishizaka, Hiroshi*

no journal, , 

no abstracts in English

Oral presentation

Induction of mutants from fragrant cyclamen "Uruwashi-no-kaori" (${it Cyclamen persicum}$ $$times$$ ${it C. purpurascens}$) by ion-beam irradiation and analysis of their flower pigments

Kondo, Emiko*; Nakayama, Masayoshi*; Kameari, Naoko*; Tanikawa, Natsu*; Morita, Yasumasa*; Kitamura, Satoshi; Akita, Yusuke; Hase, Yoshihiro; Tanaka, Atsushi; Ishizaka, Hiroshi*

no journal, , 

no abstracts in English

Oral presentation

Characteristics of yellow-flowered mutant generated from dihaploid of fragrant cyclamen (${it Cyclamen persicum}$ "Golden Boy" $$times$$ ${it C. purpurascens}$) by ion-beam irradiation

Kameari, Naoko*; Akita, Yusuke; Kitamura, Satoshi; Hase, Yoshihiro; Kondo, Emiko*; Nakayama, Masayoshi*; Kurihara, Yasushi*; Tanikawa, Natsu*; Morita, Yasumasa*; Tanaka, Atsushi; et al.

no journal, , 

no abstracts in English

Oral presentation

Molecular analysis of anthocyanin biosynthesis genes, and creation of new colored flower cultivars from fragrant cyclamen

Akita, Yusuke*; Kitamura, Satoshi; Hase, Yoshihiro; Narumi, Issey*; Ishizaka, Hiroshi*; Kondo, Emiko*; Kameari, Naoko*; Nakayama, Masayoshi*; Tanaka, Atsushi

no journal, , 

In this study, using several flower-colored mutants and their parents, we analyzed flavonoid composition in flowers by HPLC, and isolated anthocyanin biosynthetic genes by degenerate PCR. We isolated several anthocyanin biosynthetic genes from C. graecum, C. purpurascens and fragrant cyclamen cultivars. Then, we analyzed their expression patterns between wild-type and flower-colored mutants. Based on their comparison, we selected candidates for anthocyanin biosynthetic genes and confirmed their enzymatic activities in vitro. Furthermore, we also demonstrated the effectiveness of PCR-based screening method in early growth stage of mutagenized cyclamen, using newly developed DNA markers.

17 (Records 1-17 displayed on this page)
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