Visualization of DNA damage and its repair process in by pulsed-field gel electrophoresis

Kikuchi, Masahiro; Narumi, Issei; Kobayashi, Yasuhiko

The most striking feature of the radioresistant bacterium is that it can mend over 100 double-strand breaks of genomic DNA during post-irradiation incubation. This process can be clearly visualized using pulsed-field gel electrophoresis (PFGE). By a combination of protein synthesis inhibition treatment and PFGE analysis, it was possible to estimate an initial period required for induction of DNA repair proteins (induction time) and a total period required for completing DNA repair (repair time). PFGE is a powerful tool to analyze DNA damage and its repair process.

Language	:	English
Journal	:	JAERI-Conf 2002-005
Volume	:
Number	:
Pages	:	P. 185
Publication Year/Month	:	2002/03
Meeting title	:	Workshop on Recognition of DNA Damage as Onset of Successful Repair; Computational and Experimental Approaches
Held date	:	2001/12
Location (city)	:	Tokai
Location (country)	:	Japan
Patent information	:
PDF	:
Paper URL	:	https://jopss.jaea.go.jp/pdfdata/JAERI-Conf-2002-005.pdf
DOI for research data	:
Keywords	:	放射線抵抗性細菌; DNA損傷可視化; パルスフィールドゲル電気泳動; 修復時間計測; 誘導時間計測
Research Facility	:	コバルト60照射施設
Press Release	:
Article of JAEA R&D Review	:
Cooperating Institute	:

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Registration No. : B20011564
JAEA Abstracts No. :
Paper Submission No. : 25812

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