Accumulation of Cu and its oxidation state in (rusty-rock lichen) mycobiont

地衣類へのCuの取り込みとその酸化状態

藤井 洋光*; 原 光次郎*; 小峰 正史*; 尾崎 卓郎; 大貫 敏彦; 山本 好和*

Fujii, Hiromitsu*; Hara, Kojiro*; Komine, Masashi*; Ozaki, Takuo; Onuki, Toshihiko; Yamamoto, Yoshikazu*

Cu耐性地衣類へのCuの取り込みについて検討するため、培養実験を行い、Cuを濃集した地衣類試料をSEM-EDS分析及びXANES分析により分析した。その結果、Cu(II)が細胞表面に分布し、細胞内に取り込まれたCuはCu(I)に還元していることを明らかにした。

We studied the association of a Cu tolerant mycobiont, Tremolecia atrata (rusty-rock lichen) with Cu. T. atrata mycobiont cell aggregates, which grew into a sperical shape, rapidly absorbed Cu into their inner and outer parts. The EDS/SEM study showed that Cu was more highly accumulated in the inner part than the outer part of the aggregates. The XANES study revealed that the Cu absorbed by the T. atrata mycobiont was both monovalent and divalent. These results suggested that the T. atrata mycobiont's high tolerance to Cu is attributable to its ability to store Cu(I) inside the cytoplasm and to adsorb Cu(II) on the cell wall.