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Report No.

Extraction of cytochrome c by a functionalized ionic liquid containing a crown ether

Kubota, Fukiko*; Koyanagi, Yusuke*; Nakashima, Kazunori*; Shimojo, Kojiro   ; Kamiya, Noriho*; Goto, Masahiro*

We carried out the extraction of a protein cytochrome c (Cyt-c) with an ionic liquid with an attached crown ether moiety in the imidazolium cation. The functional ionic liquid, [18C6mim][PF$$_{6}$$], was dissolved in two different ionic liquids as diluents, one having a hydroxyethyl group, [C$$_{2}$$OHmim][Tf$$_{2}$$N], and the other an ethyl group, [C$$_{2}$$mim][Tf$$_{2}$$N], respectively. The transfer of Cyt-c from the aqueous phase to the ionic liquid phase took place with [18C6mim][PF$$_{6}$$] in the [C$$_{2}$$OHmim][Tf$$_{2}$$N] system. Although the extraction ability was lower than that of dicyclohexano-18-crown-6 (DCH18C6), the functionalized ionic liquid system gave a better stripping performance than the ordinary crown ether system. The extraction efficiency was not influenced by operating temperature when the concentration of [18C6mim] was high whereas it was greatly affected by temperature at low [18C6mim] concentration.



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