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Report No.
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Isolation and characterization of alkaliphilic nitrate-reducing bacteria from soil

Murakami, Hiroshi; Kato, Takashi*; Honda, Akira

As part of the research for estimating the impact of nitrate on the safety of a repository for radioactive wastes, the isolation and characterization of nitrate-reducing bacteria in alkaline conditions were done. The isolated bacteria were classified into three species. The bacteria showed nitrate-reducing activity at pH 10 and one of them displayed an alkaliphilic nature. As this is a generic study, the most common species among the isolated bacteria was studied to determine the optimal conditions in terms of temperature, pH and concentration of electron receptor for nitrate reduction.

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