Protein expression of in response to uranium exposure

Sakamoto, Fuminori  ; Nankawa, Takuya ; Kozai, Naofumi   ; Fujii, Tsutomu*; Iefuji, Haruyuki*; Francis, A. J.; Onuki, Toshihiko

Protein expression of grown in the medium containing U(VI) and U(VI) was examined by two-dimensional gel electrophoresis. of BY4743 was grown in medium containing U of 0, 2.0, and 5.0 10 M or U of 2.5 10 (radioactivity was higher by 350 times than 2.0 10 M U) and 5.0 10 M for 112 h at 30C. The growths of the yeast grown in the above media were in the following order: control 2.5 10 M U 2.0 10 M U 5.0 10 M U 5.0 10 M U. This result indicated that not only radiological but also chemical effect of U reduced the growth of the yeast. The concentrations of U in the medium containing U or U decreased, suggesting U accumulation by the yeast cells. The 2-D gel electrophoresis analysis showed the appearance of several spots after exposure to U or to U but not in the control containing no uranium. These results show that the yeast cells exposed to U express several specific proteins.