Application of real time PCR for the quantitative detection of radiation-induced genomic DNA strand breaks

Yamauchi, Emiko*; Watanabe, Ritsuko; Oikawa, Miyoko*; Fujimoto, Hirofumi*; Yamada, Akinori*; Saito, Kimiaki ; Murakami, Masahiro*; Hashido, Kazuo*; Tsuchida, Kozo*; Takada, Naoko*; Fugo, Hajime*; Tu, Z.*; Maekawa, Hideaki*

The frequency of single strand breaks (SSBs) occurring on both strands of the pBR322 plasmid DNA region flanked by a pair of primers used for polymerase chain reaction (PCR) amplifications was determined after irradiation with $^{137}$ Cs -rays. We refined that real time PCR is suitable for the detection and quantitative analysis of SSBs caused by -ray irradiation. The utility of this approach was also supported by the comparison of the practical experimental data with the Monte Carlo simulation. The potential application of this PCR method for the detection of genomic DNA damage was also confirmed.

Language	:	English
Journal	:	Journal of Insect Biotechnology and Sericology
Volume	:	77
Number	:	1
Pages	:	p.17 - 24
Publication Year/Month	:	2008/02
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Registration No. : AA20080076
JAEA Abstracts No. : 36000811
Paper Submission No. : 4133

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