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Report No.

Kinetic analysis of double-strand break rejoining reveals the DNA reparability of $$gamma$$-irradiated tobacco cultured cells

Yokota, Yuichiro; Wada, Seiichi*; Hase, Yoshihiro; Funayama, Tomoo; Kobayashi, Yasuhiko; Narumi, Issei; Tanaka, Atsushi

The rejoining efficiency of double-strand breaks (DSBs) was quantified in tobacco protoplasts and CHO-K1 cells following $$gamma$$-ray irradiation in order to compare DNA reparability of higher plants with mammals. The DSB rejoining kinetics of tobacco protoplasts were well represented by a biphasic-exponential equation: half of initial-induced DSBs were rejoined for 1 h and the others were almost rejoined within 4 h. We found that the DSB rejoining kinetics of tobacco protoplasts at 27$$^{circ}$$C are the same as those of CHO-K1 cells at 37$$^{circ}$$C. These findings indicate that the DSB rejoining efficiency of tobacco protoplasts and CHO-K1 cells are comparable at their respective cell cultivation temperatures, suggesting that DSB rejoining efficiency is little responsible for the higher radiation-tolerance of tobacco protoplasts.



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