Imaging of translocation of Zn from the roots to the shoots in Zn/Cd hyperaccumulator by PETIS

Sekimoto, Hitoshi*; Kawachi, Naoki; Watanabe, Satoshi; Yamaguchi, Yoshie*; Ozawa, Toyoaki*; Shima, Daisuke*; Ishikawa, Sayuki*; Fujimaki, Shu; Suzui, Nobuo; Ishii, Satomi; Ishioka, Noriko; Suto, Hiroyuki*; Matsuhashi, Shimpei

The heavy metal hyperaccumulation phenotype seen in is the result of physiological differences at the cellular, organ and whole plant levels relative to non-hyperaccumulator plant species. These differences in Zn and Cd content relative to non-hyperaccumlators is due, in part, to enhanced metal transport at a number of different sites within the plant, including the root surface, xylem loading and reabsorption of xylem-born metals by leaf cells. In the present study, the taranslocation of Zn from root to the shoots in (hyperaccumulator) relative to (non-hyperaccumlator) was examined by the positron-emitting tracer imaging system (PETIS). The serial images of translocation of Zn from the roots to the shoots were acquired successfully, and the time activity curves for translocation of Zn in the basal shoot and the root region were analyzed in the two plants set in the same field of view. It was indicated that the translocation of Zn from the root to the shoot of , that is a Zn/Cd-hyperaccumulator, would be enhanced.