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Report No.

Crystal structure of glycosyltrehalose synthase from Sulfolobus shibatae DSM5389

Okazaki, Nobuo; Tamada, Taro; Kato, Masaru*; Miura, Yutaka*; Kobayashi, Kazuo*; Kuroki, Ryota

We successed crystallization of glycosyltransferase (GTSase) from Sulfolobus shibatae DSM5389, and determined crystal structure with data which was collected at SPring-8 BL41XU. The enzyme has 729 amino acids and five domains. Domain A is major domain which has ($$beta$$/$$alpha$$)8 barrel catalytic domain as in the usual alpha-amylase family enzymes. The catalytic center is located in the center of the ($$beta$$/$$alpha$$)8 barrel. In the near ($$beta$$/$$alpha$$)8 barrel, electric density of Mg$$^{2+}$$ ion is observed. Some hydrogen bonding network is observed, too.



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