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Isolation and characterization of suppressors of the ${it early flowering 3}$ in ${it Arabidopsis thaliana}$

Natsui, Yu*; Nefissi, R.*; Miyata, Kana*; Oda, Atsushi*; Hase, Yoshihiro; Nakagawa, Mayu*; Mizoguchi, Tsuyoshi*

EARLY FLOWERING 3 (ELF3) is a circadian clock protein with a major role in maintaining circadian rhythms in plants. In this work, ${it elf3-101}$ was mutagenized by EMS in plants of the Lansberg ${it erecta}$ background to isolate suppressors of ${it elf3}$ and to understand the molecular mechanisms of flowering time controlled by ELF3. Two suppressors, ${it sel106}$ obtained from this screen and ${it sel5}$ from a precious study, were chosen for further analysis. Genetic mapping, gene expression analysis, and sequencing identified ${it sel106}$ and ${it sel5}$ as new alleles of ${it gi}$ and ${it fca}$, respectively. Genetic interactions between ${it elf3}$ and ${it gi}$ and between ${it elf3}$ and ${it fca}$ in the control of the floral activator ${it FLOWERING LOCUS T}$ were also investigated. Six suppressor of ${it elf3}$ were classified at least into four subgroups based on the expression of such floral regulators as GI, CO, FT, SVP, and FLC, and on their flowering times under LL, LD, and SD. This classification scheme is useful for the characterization of unidentified suppressor mutations.

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Category:Biotechnology & Applied Microbiology

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