Functional analysis of flavonoid accumulation genes of

シロイヌナズナのフラボノイド蓄積遺伝子の機能解析

北村 智; 松田 史生*; 峠 隆之*; 榊原 圭子*; 山崎 真巳*; 斉藤 和季*; 鳴海 一成

Kitamura, Satoshi; Matsuda, Fumio*; Toge, Takayuki*; Sakakibara, Keiko*; Yamazaki, Mami*; Saito, Kazuki*; Narumi, Issei

Flavonoids are one of the most widely found secondary metabolites in plants. We previously isolated ion-beam-induced Arabidopsis mutant , which showed aberrant accumulation pattern of anthocyanins and proanthocyanidins (PAa). To address the flavonoid transport mechanism more deeply, flavonoid accumulation mutants of were analyzed at the cell biological and metabolomic levels. Within PA-accumulating cells, GFP-TT19 proteins were detected in the cytosol. This suggests that TT19 functions before vacuolar uptake of flavonoids. Metabolic profiling demonstrated that PA derivatives such as epicatechins and epicatechin oligomers, although highly accumulated in wild-type, were absent in . Based on the metabolic and cytological characteristics of mutants, we proposed a model concerning the PA pathway.