中性子小角散乱; 応用編

Small-angle neutron scattering for biology; Application of SANS to biological study

遠藤 仁; 杉山 正明*; 井上 倫太郎*

Endo, Hitoshi; Sugiyama, Masaaki*; Inoue, Rintaro*

中性子小角散乱法を用いたタンパク質の高次構造解析法及び中性子スピンエコー法を用いたタンパク質のダイナミクス解析に関して、著者らの最新の研究例を用いて解説した。例として、「タンパク質ミネラル複合粒子の微細構造解析」、「重水素化タンパク質を用いたSubunitの交換現象の解析」、「ホスホグリセリン酸キナーゼの内部運動解析」の3つの研究成果を用いた。

In this text, the authors introduce two powerful techniques and one developing one for Small-Angle Neutron Scattering (SANS). The most fascinating feature of neutron as a scattering probe is its isotope effect in hydrogen. The first topic is concerning about recent progress on Contrast Variation Method: the author shows how to apply the contrast variation method to protein-mineral complex system and analyze the data. The second is concerning about deuteration-labeling: the author shows kinetics analysis in quaternary structure of homo-oligomeric protein with this technique. The final topic is concerning about the next generation analysis: an analysis method coupling SANS with neutron spin echo for dynamics of tertiary structure of protein.