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Report No.

Estimation of heavy-ion-induced bystander effects between lung normal and cancer cells

Ikeda, Hiroko; Yokota, Yuichiro; Funayama, Tomoo; Kanai, Tatsuaki*; Kobayashi, Yasuhiko

The purpose of this research is to elucidate comprehensively what kind of influence heavy-ion-induced bystander effects have in cancer therapy. In our experiments, human lung normal fibroblasts WI-38 line and human lung cancer cells H1299/wt${it p53}$ line were used. Cells were irradiated with carbon-ion broad beams (WI-38: 0.13 Gy, H1299/wt${it p53}$: 0.5 Gy), then survival rates of bystander cells after 6- or 24-hours co-culture with irradiated cells was calculated using colony formation assay. When we co-cultured irradiated WI-38 with non-irradiated WI-38, it was found survival rates of WI-38 decreased about 10%-15% after 6-hours and 24-hours co-culture. On the other hand, when we co-cultured irradiated H1299/wt${it p53}$with non-irradiated WI-38, it was found that survival rates of WI-38 increased about 10% after 24-hours co-culture. Consequently, it was thought that survival rates of non-irradiated cells were increased by co-culture between different type cells.



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