Effect of carbon ion beam irradiation on callus growth in Arabidopsis and rice

Saika, Hiroaki*; Yoshihara, Ryohei*; Sakamoto, Ayako; Toki, Seiichi*

To date, many commercial varieties of various kinds of plants have been developed by ion beam breeding. However, to further improve the ion beam breeding system, it is important to gather more basic information on the mutagenic effects of ion beams in plants. To this end, we try to establish a novel mutation detection system using a conditional negative selection marker to stop cell growth. Cultured cell such as callus that can clonally propagate efficiently and easily are well-suited to this system. In this study, we checked the sensitivity of callus of the model plants Arabidopsis and rice to carbon ion beams.