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Effects of ion beam radiation on genome integrity in ${it Saccharomyces cerevisiae}$

Nunoshiba, Tatsuo*; Hirasawa, Yukei*; Yamauchi, Ayako*; Sato, Katsuya; Tejima, Kohei*; Onodera, Takefumi; Narumi, Issei

The LOH (loss of heterozygosity) induction by various ion beam radiations was investigated in diploid ${it Saccharomyces cerevisiae}$. The ion beams $$^{12}$$C$$^{5+}$$, $$^{12}$$C$$^{6+}$$, $$^{4}$$He$$^{2+}$$ and $$^{40}$$Ar$$^{13+}$$ at the lowest dose (75, 75, 200 and 150 Gy, respectively), which had 30-50% lethality, induced LOH with 10-fold higher frequency. The LOH induction was predominantly ($$sim$$90%) resulted from gene conversion or crossover through homologous recombination, and followed by chromosome loss through chromosome mis-segregation. Induction of LOH by $$^{12}$$C$$^{5+}$$ was also examined in ${it S. cerevisiae}$ deficient in AP endonuclease (Apn1), translesion DNA polymerases Pol$$eta$$ encoded by Rad30 and Pol$$zeta$$ encoded by Rev3 and Rev7. Pol$$zeta$$ may be involved in the induction of LOH, because a slight induction of LOH by $$^{12}$$C$$^{5+}$$ was observed in the Rev3-deficient strain.



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