ESRスピントラップ法による放射線照射漢方エキス剤のラジカル捕捉活性評価

Measurement of radical scavenging activity of irradiated Kampo extracts using ESR spin-trap method

太多 由依*; 川村 翔栄*; 菊地 正博; 小林 泰彦; 鵜飼 光子*; 中村 秀夫*

Ota, Yui*; Kawamura, Shoei*; Kikuchi, Masahiro; Kobayashi, Yasuhiko; Ukai, Mitsuko*; Nakamura, Hideo*

100kGy放射線照射及び未照射のそれぞれ13種類の漢方エキス剤を用い、新規ラジカル試薬CYPMPOを用いたESRスピントラップ法によりラジカル捕捉活性を計測した。複数のエキス剤でアルコキシラジカルやヒドロキシラジカルに対する高いラジカル捕捉活性を確認した。100kGy放射線照射により、いくつかの漢方エキス剤のラジカル捕捉活性が変化した。ESRスピントラップ法を用いて、放射線照射による漢方エキス剤のラジカル捕捉活性への影響を評価できた。

The radical scavenging activity (RSA) of 13 kinds of -ray irradiated Kampo extracts were studied by ESR spin-trap method. The RSA against alkoxy radical and hydroxyl radical were measured using new spin trapping reagent CYPMPO. The RSA against these two radicals were evaluated using GSH for alkoxy RSA and L-ascorbic acid for hydroxy RSA as a standard antioxidant reagent. We revealed that a few Kampo extracts showed high RSA against alkoxy radical and hydroxy radical. This RSA of Kampo extracts was changed by -ray irradiation treatment. Using ESR spin-trap method, it is concluded that the effect of radiation treatment on RSA of Kampo extracts were able to detect.