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Effect of HisTag on the secretion of cellulase EGPf by brevibacillus expression system

Shimizu, Rumi; Hiromoto, Takeshi; Adachi, Motoyasu; Kuroki, Ryota; Kataoka, Misumi*; Ishikawa, Kazuhiko*

For neutron crystal structure analysis of proteins, it is necessary to prepare large crystals in volume (several mm$$^{3}$$) compared to that required for X-ray crystal structure analysis. To prepare the large volume crystal, we inevitably need much amount of purified protein. As a development in preparation of samples for neutron crystal structure analysis of proteins, we have tried to develop expression system for many kinds of protein using Eschericha coli, Brevibacillus, Pichia pastoris, cultivation cell and so on. Recently, we have succeeded in high level expression of cellulose (EGPf) derived from Archaea ${it Pyrococcus furiosus}$ using Brevibacillus system.



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