検索対象:     
報告書番号:
※ 半角英数字
 年 ~ 
 年

Detection and measurement of abnormal posture under stressful conditions in nematode $textit{C. elegans}$

ストレス条件下の線虫$textit{C. elegans}$の姿勢異常の検出と計測

Ide, Kazunori*; Ishikawa, Tomoya*; Arai, Shingo*; Morioka, Tatsuya*; Kaneda, Hiroki*; 鈴木 芳代; 坂下 哲哉; 石井 直明*; 簗瀬 澄乃*

Ide, Kazunori*; Ishikawa, Tomoya*; Arai, Shingo*; Morioka, Tatsuya*; Kaneda, Hiroki*; Suzuki, Michiyo; Sakashita, Tetsuya; Ishii, Naoaki*; Yanase, Sumino*

Oxidative stress threatens cellular and extracellular targets including lipids, proteins and nucleic acids. As a result, oxidative stress causes or influence apoptosis, degenerative diseases and aging. Not only that, we found an abnormal posture in short-lived mutant such as $textit{mev-1}$, which is a paraquat (methylviologen)-sensitive strain, exposed to hyperoxia. In this study, we detected the hyperoxia-induced abnormal posture in $textit{mev-1}$ mutant compared with wild-type N$$_{2}$$ and long-lived $textit{age-1}$ strains. 4-day-old animals placed on a NGM agar plate with a fixed bacterial lawn were exposed to hyperoxia (90% of oxygen) overnight. After the exposure, some animals were immediately recorded as digital images with up to 400-fold magnification of the microscope. The images were analyzed using image processing software, Wriggle Tracker and Move-tr/2D, by a previously published method (Hattori et al., 2012). The body line was skeletonized and evenly divided into 12 segments. Subsequently, X- and Y-coordinates of each point on the body were acquired. To evaluate the posture of the body, we introduced a novel standard, namely the "posture index" as the absolute average of the relative angle, $$q_i$$, between adjacent dividing points. As a result of this investigation, we can not only intuitively but also quantitatively and statistically understand a tendency of the behavior in a short-lived mutant under stressful conditions.

Access

:

- Accesses

InCites™

:

Altmetrics

:

[CLARIVATE ANALYTICS], [WEB OF SCIENCE], [HIGHLY CITED PAPER & CUP LOGO] and [HOT PAPER & FIRE LOGO] are trademarks of Clarivate Analytics, and/or its affiliated company or companies, and used herein by permission and/or license.