Difference in dynamics between F-actin and myosin S1 measured by neutron scattering

Matsuo, Tatsuhito; Arata, Toshiaki*; Oda, Toshiro*; Fujiwara, Satoru

The dynamics of F-actin and myosin S1 were studied by quasi-elastic neutron scattering (QENS). QENS measurements were conducted on D $_{2}$ O solution samples of F-actin and S1 at 300 K using AMATERAS in J-PARC. The QENS spectra of proteins were obtained by subtracting those of D $_{2}$ O buffer from those of D $_{2}$ O samples. In the current analysis, it was found that while the correlation time of atomic motions of S1 was similar to that of other proteins studied so far, the correlation time of F-actin was shorter than S1. Furthermore, F-actin had a population of the atoms undergoing diffusive motions with larger amplitudes than S1. These results suggest that F-actin is more flexible than other proteins including S1.

Language	:	English
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Meeting title	:	日本生物物理学会第52回年会
Held date	:	2014/09
Location (city)	:	札幌
Location (country)	:	日本
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Keywords	:	タンパク質ダイナミクス; 中性子非弾性散乱
Research Facility	:	J-PARC物質・生命科学実験施設
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Registration No. : BB20141736
JAEA Abstracts No. :
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