Screening of salt tolerant mutants regenerated from irradiated stem segments of Lombardy poplar ()

セイヨウハコヤナギの放射線照射茎片から再分化させた耐塩性変異体の選抜

Biswas, K. K.*; 長谷 純宏; 大野 豊

Biswas, K. K.*; Hase, Yoshihiro; Ono, Yutaka

Species of the genus are ecologically important trees and often serving as a vegetative pioneer species because of their fast growth rates. The rapid growth of poplar is dependent on access to sufficient water in plantation sites and this is problematic. To overcome this problem, a forward genetics approach has been taken for developing salt/drought tolerant mutants by combination of radiation mutagenesis and in vitro regeneration in . Previously we screened regenerated shoots regenerated from stems exposed with cobalt-60 source of rays and isolated two mutant candidates named and . Only about 25% stem segments of both lines produced roots in RIM with 75 mM NaCl. The low frequency in root production in NaCl medium may be attributed to that these plants are chimeric. We also successfully obtained at least 2 salt tolerant candidates regenerated from stem segments irradiated with 320 MeV C ion beams.