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Molecular analysis of polyphosphate biosynthesis-related genes in ${it Deinococcus radiodurans}$

放射線抵抗性細菌におけるポリリン酸生合成関連遺伝子の解析

上田 涼史郎; 佐藤 勝也; 林 秀謙*; 鳴海 一成*; 大野 豊

Ueda, Ryoshiro; Sato, Katsuya; Hayashi, Hidenori*; Narumi, Issey*; Ono, Yutaka

It is suggesting a possibility that the polyphosphate granules in cells could accumulate harmful metal ions such as radiocesium to protect the cells from cytotoxicity. ${it Deinococcus radiodurans}$ has been considered as a microorganism for bioremediation under highly radioactive contaminated environments. We generated the polyphosphate biosynthesis-related genes (${it ppk}$ and ${it ppx}$) disruptant strains and characterized their disruption effect to clarify the role of polyphosphate for the accumulation of cesium in ${it D. radiodurans}$. The intracellular levels of polyphosphates in the ${it ppk}$ disruptant and overexpressing strains were almost same and slightly increased, respectively. On other hand, the intracellular level of polyphosphates significantly increased in the ${it ppx}$ disruptant strains, suggesting that the ${it ppx}$gene plays an important role in the accumulation of polyphosphate in ${it D. radiodurans}$.

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