Analysis of bystander effect induced by cell membrane response in glioma cells

Wada, Seiichi*; Ando, Tatsuhiko*; Watanabe, Aya*; Kakizaki, Takehiko*; Natsuhori, Masahiro*; Funayama, Tomoo; Sakashita, Tetsuya; Yokota, Yuichiro; Kobayashi, Yasuhiko

So far, we clarified that X-ray irradiation induced cell killing by bystander effect mediated-secreted factor. This phenomenon was related with sphingomyelinase (SMase). In this study we analyzed mechanism of secreted SMase from irradiated cells after irradiation. SMase was detected in the culture medium after irradiation by SDS-PAGE. Then, SMase was detected in the exosome of culture medium, but not out of exosome after irradiation. This result indicates that SMase was secreted as exosome from the irradiated cells.

Language	:	English
Journal	:	JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013
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Pages	:	P. 79
Publication Year/Month	:	2015/03
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Paper URL	:	https://doi.org/10.11484/jaea-review-2014-050
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Keywords	:	重イオンビーム; バイスタンダー効果; 細胞膜応答
Research Facility	:	TIARA(イオン照射研究施設)
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Article of JAEA R&D Review	:
Cooperating Institute	:	北里大学

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Registration No. : AA20150026
JAEA Abstracts No. :
Paper Submission No. : 17471

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