Screening of cesium-accumulating mutant of radioresistant bacterium by ion beam breeding technology

イオンビーム育種技術による放射線抵抗性細菌デイノコッカス・ラジオデュランスのセシウム蓄積変異株の選抜

佐藤 勝也; 上田 涼史郎*; 長谷 純宏; 鳴海 一成*; 大野 豊

Sato, Katsuya; Ueda, Ryoshiro*; Hase, Yoshihiro; Narumi, Issey*; Ono, Yutaka

Bioremediation uses biological organisms to solve an environmental problem. However, microorganisms are sometimes not enough effective to achieve efficient recovery. Therefore, enhancing the functionality of microorganisms is needed to promote the active use of bioremediation. This study began with the purpose of development of Cs-accumulating bacteria by ion beam breeding technology. Previously, we suggested that is the most suitable species for the development of high Cs-accumulating bacteria by ion beam breeding technology. Candidates of Cs-accumulating mutant were screened from the mutagenized cells by ion beam breeding technology on the basis of the luminescent intensity and the cell area as indicators of intracellular Cs level and cell growth, respectively. As a result, we obtained 33 candidates, whose Cs-accumulating abilities were over 2-fold higher than that of the wild-type, from the population of mutagenized cells.