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Report No.

Improvement of symbiotic performance of ${it Sinorhizobium}$ with ${it Vigna marina}$ under high salt conditions using the ion beam mutation breeding

Maruyama, Yudai*; Takeda, Kiyoko*; Tomooka, Norihiko*; Sato, Katsuya; Ono, Yutaka; Yokoyama, Tadashi*

${it Vigna marina}$ is one of wild legumes, which called as Hamaazuki, are distributed at the vicinity of the coast from tropical to sub-tropical regions and have high salt tolerance among terrestrial plants. ${it V. marina}$ can grow at 400 mM NaCl condition. Furthermore, the root nodules on ${it V. marina}$ have a symbiotic relationship with rhizobia that related to the genus ${it Sinorhizobium}$ having high stress tolerance (500 mM NaCl, 45 $$^{circ}$$C, pH 10.5). Above both symbiotic partners showed extremely high salt tolerance, however, ${it V. marina}$ cannot develop root nodules with ${it Sinorhizobium}$ at over 80 mM NaCl conditions. To improve symbiotic performance of ${it V. marina}$ with ${it Sinorhizobium}$ under high salt conditions, ${it Sinorhizobium}$ bacterial cells were irradiated by carbon ion-beam at TIARA. However, so far, we could not obtain promising mutants having good symbiotic performance with Vigna marina under high salt conditions, yet.



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