メダカの群れ行動解析に向けた画像バイオロギング

Video bio-logging for behavior analysis of medaka in a group

松田 朝陽*; 高橋 悟*; 川端 邦明   ; 尾田 正二*; 金子 俊一*

Matsuda, Asahi*; Takahashi, Satoru*; Kawabata, Kuniaki; Oda, Shoji*; Kaneko, Shunichi*

メダカの生態、特に、群行動に関する行動解析を支援するために、動画像からメダカを位置を検出し、遊泳軌跡を記録するための手法について開発を行った。開発手法の説明と実験により得られた結果について報告する。

Medaka (Oryzias latipes) has genetic diversity, and analyzing its behavior in a group is very important for elucidation of diversity of organisms. However, conventional method of visual measurement has some problems. For example, the time cost and human error. In this paper, we proposed a method of behavior measurement of medaka in a group based on an image instrumentation. Our proposed method consists of four processes: pre-processing, particle filter, orientation code matching using by central differences with multi-templates, and detection of target position. Finally, we examined the effectiveness of our proposed method through an experimentation.