ヒトiPS細胞由来組織幹細胞における放射線依存的突然変異解析

Analysis of radiation induced mutation in organ cells derived from human induced pluripotent stem cells

島田 幹男*; 神崎 訓枝   ; 柳原 啓見*; 三宅 智子*; 松本 義久*

Shimada, Mikio*; Kanzaki, Norie; Yanagihara, Hiromi*; Miyake, Tomoko*; Matsumoto, Yoshihisa*

突然変異頻度は組織の種類と分化レベルに依存するが不明な点が多い。本研究では、組織別の放射線影響を解析するため、ヒトiPS細胞から、神経,皮膚,心筋,血液の4つの異なる組織細胞を作製した。人工知能技術と機械学習法を使用して、組織ごとの突然変異頻度の違いを比較検討した結果を報告する。

Although mutation frequency depends on organ cell types and differentiation level, it is not fully understood that organ cell types dependent mutation frequency in human cells. In this study, we aimed to establish measurement system of radiation dependent mutation frequency for analyze radiation effect to the human body. For this purpose, we derived four different organ cells such as neural cells, skin keratinocytes, heart muscle cells and blood cells from hiPSCs. Further, using artificial intelligence technology and machine leaning method, we w analyzed differences of mutation frequency during samples.