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Kikuchi, Masahiro; Gunawardane, C. R.*; Alam, M. K.*; Mohd Dzomir, A. Z.*; Pitipanaarachchi, R. C.*; Funayama, Tomoo; Hamada, Nobuyuki*; Sakashita, Tetsuya; Kobayashi, Yasuhiko
JAEA-Review 2008-055, JAEA Takasaki Annual Report 2007, P. 88, 2008/11
no abstracts in English
Kikuchi, Masahiro; Gunawardane, C. R.; Alam, M. K.; Mohd Dzomir, A. Z.; Pitipanaarachchi, R. C.; Funayama, Tomoo; Hamada, Nobuyuki*; Sakashita, Tetsuya; Wada, Seiichi*; Sato, Katsuya; et al.
Radioisotopes, 56(9), p.509 - 517, 2007/09
Since ionizing radiation is used for sterilizing or lowering the microbial content of foods, the development of versatile detection methods of irradiated foods is necessary for appropriate management. In an effort to distinguish between irradiated and non-irradiated food, a method based on the detection of oxidative DNA base damage using the chemiluminescence enzyme-linked immunosorbent assay (ELISA) with anti-8-hydroxy-2'-deoxyguanosine antibody was developed. It was shown that this chemiluminescence ELISA method could be applied to chicken, beef and pork that were irradiated with over 3 kGy. Twenty milligrams of a loaf of meat was sufficient to distinguish between irradiated and non-irradiated meat by this method.
Kikuchi, Masahiro; Mohd Dzomir, A. Z.; Gunawardane, C. R.; Alam, M. K.; Kobayashi, Yasuhiko
JAEA-Review 2006-042, JAEA Takasaki Annual Report 2005, P. 72, 2007/02
Ionizing radiation has been used for reducing food losses and securing food safety. The development of versatile detection methods of irradiated foods is needed for appropriate management. In an effort to distinguish between irradiated and non-irradiated meats, a detection method of oxidative DNA base damages using chemiluminescence enzyme-linked immunosorbent assay with anti-8-hydroxy-2'-deoxyguanosine antibody was developed. The procedure of this method is not so complicated, and relatively short time is needed to obtain results without special techniques. For most of all foods containing DNA, this novel method should be applicable to detect the damaged DNA of irradiated foods. Application to beef meat irradiated with 3 kGy or higher was suggested to be detectable successfully.
Kikuchi, Masahiro; Gunawardane, C. R.*; Alam, M. K.*; Kobayashi, Yasuhiko
no journal, ,
no abstracts in English
Kikuchi, Masahiro; Gunawardane, C. R.*; Alam, M. K.*; Kobayashi, Yasuhiko
no journal, ,
no abstracts in English
Kikuchi, Masahiro; Mohd Dzomir, A. Z.; Gunawardane, C. R.; Alam, M. K.; Kobayashi, Yasuhiko
no journal, ,
no abstracts in English
