Cross-scale analysis of temperature compensation in the cyanobacterial circadian clock system

Furuike, Yoshihiko*; Ouyang, D.*; Tominaga, Taiki*; Matsuo, Tatsuhito*; Mukaiyama, Atsushi*; Kawakita, Yukinobu; Fujiwara, Satoru*; Akiyama, Shuji*

Communications Physics (Internet), 5(1), p.75_1 - 75_12, 2022/04

https://doi.org/10.1038/s42005-022-00852-z

Segmental motions of proteins under non-native states evaluated using quasielastic neutron scattering

Fujiwara, Satoru*; Matsuo, Tatsuhito*; Sugimoto, Yasunobu*; Shibata, Kaoru

Journal of Physical Chemistry Letters (Internet), 10(23), p.7505 - 7509, 2019/12

https://doi.org/10.1021/acs.jpclett.9b03196

Characterization of the dynamics of disordered polypeptide chains is required to elucidate the behavior of intrinsically disordered proteins and proteins under non-native states related to the folding process. Here we develop a method using quasielastic neutron scattering, combined with small-angle X-ray scattering and dynamic light scattering, to evaluate segmental motions of proteins as well as diffusion of the entire molecules and local side-chain motions. We apply this method to RNase A under the unfolded and molten-globule (MG) states. The diffusion coefficients arising from the segmental motions are evaluated and found to be different between the unfolded and MG states. The values obtained here are consistent with those obtained using the fluorescence-based techniques. These results demonstrate not only feasibility of this method but also usefulness to characterize the behavior of proteins under various disordered states.

Revising the 4 symmetry in CeCuGe; Soft X-ray absorption and hard X-ray photoemission spectroscopy

Aratani, Hidekazu*; Nakatani, Yasuhiro*; Fujiwara, Hidenori*; Kawada, Moeki*; Kanai, Yuina*; Yamagami, Kohei*; Fujioka, Shuhei*; Hamamoto, Satoru*; Kuga, Kentaro*; Kiss, Takayuki*; et al.

Physical Review B, 98(12), p.121113_1 - 121113_6, 2018/09

AA2018-0352.pdf:1.16MB

https://doi.org/10.1103/PhysRevB.98.121113

Dynamical behavior of human -synuclein studied by quasielastic neutron scattering

Fujiwara, Satoru; Araki, Katsuya*; Matsuo, Tatsuhito; Yagi, Hisashi*; Yamada, Takeshi*; Shibata, Kaoru; Mochizuki, Hideki*

PLOS ONE (Internet), 11(4), p.e0151447_1 - e0151447_17, 2016/04

https://doi.org/10.1371/journal.pone.0151447

Structures of the troponin core domain containing the cardiomyopathy-causing mutants studied by small-angle X-ray scattering

Matsuo, Tatsuhito; Takeda, Soichi*; Oda, Toshiro*; Fujiwara, Satoru

Biophysics and Physicobiology (Internet), 12, p.145 - 158, 2015/12

http://doi.org/10.2142/biophysico.12.0_145

Accurate structure analyses of polymer crystals on the basis of wide-angle X-ray and neutron diffractions

Tashiro, Koji*; Hanesaka, Makoto*; Yamamoto, Hiroko*; Wasanasuk, K.*; Jayaratri, P.*; Yoshizawa, Yoshinori*; Tanaka, Ichiro*; Niimura, Nobuo*; Kusaka, Katsuhiro*; Hosoya, Takaaki*; et al.

Kobunshi Rombunshu, 71(11), p.508 - 526, 2014/11

https://doi.org/10.1295/koron.71.508

The crystal structure analysis of various polymer substances has been reviewed on the basis of wide-angle high-energy X-ray and neutron diffraction data. The progress in structural analytical techniques of polymer crystals have been reviewed at first. The structural models proposed so far were reinvestigated and new models have been proposed for various kinds of polymer crystals including polyethylene, poly(vinyl alcohol), poly(lactic acid) and its stereocomplex etc. The hydrogen atomic positions were also clarified by the quantitative analysis of wide-angle neutron diffraction data, from which the physical properties of polymer crystals have been evaluated theoretically. The bonded electron density distribution has been estimated for a polydiacetylene single crystal on the basis of the so-called X-N method or by the combination of structural information derived from X-ray and neutron diffraction data analysis. Some comments have been added about future developments in the field of structure-property relationship determination.

Coupling of the hydration water dynamics and the internal dynamics of actin detected by quasielastic neutron scattering

Fujiwara, Satoru; Plazanet, M.*; Oda, Toshiro*

Biochemical and Biophysical Research Communications, 431(3), p.542 - 546, 2013/02

https://doi.org/10.1016/j.bbrc.2013.01.021

Dynamics of cardiomyopathy-causing mutant of troponin measured by neutron scattering

Matsuo, Tatsuhito; Natali, F.*; Plazanet, M.*; Zaccai, G.*; Fujiwara, Satoru

Journal of the Physical Society of Japan, 82(Suppl.A), p.SA020_1 - SA020_5, 2013/01

https://doi.org/10.7566/JPSJS.82SA.SA020

Troponin is a protein that regulates the muscle contraction depending on the intracellular Ca concentration. K247R mutation of TnT is known to cause the hypertrophic cardiomyopathy. In this work, neutron scattering was used to detect possible changes in dynamics of troponin caused by mutation. Elastic incoherent neutron scattering experiments were carried out on solution samples of the wild type, and K247R mutant at the IN13 spectrometer at the Institut Laue-Langevin, at temperatures between 280 K and 292 K with an interval of 3 K. From the measured scattering data, force constants (k), which reflect the resilience of the protein, were calculated. The k values for the wild type and K247R mutant were 0.077 (0.035) N/m and 0.046 (0.026) N/m (mean(s.d.)), respectively. This suggests that the disease-causing mutant is more flexible than the wild type. The large flexibility might modulate Ca signal transmission mechanism, leading to the functional aberration.

Repetition Rate Multiplication: RRM, an advanced measuring method planed for the backscattering instrument, at the MLF, J-PARC

Takahashi, Nobuaki; Shibata, Kaoru; Kawakita, Yukinobu; Nakajima, Kenji; Inamura, Yasuhiro; Nakatani, Takeshi; Nakagawa, Hiroshi; Fujiwara, Satoru; Sato, Taku*; Tsukushi, Itaru*; et al.

Journal of the Physical Society of Japan, 80(Suppl.B), p.SB007_1 - SB007_4, 2011/12

https://doi.org/10.1143/JPSJS.80SB.SB007

Identified charged hadron production in collisions at = 200 and 62.4 GeV

Adare, A.*; Afanasiev, S.*; Aidala, C.*; Ajitanand, N. N.*; Akiba, Yasuyuki*; Al-Bataineh, H.*; Alexander, J.*; Aoki, Kazuya*; Aphecetche, L.*; Armendariz, R.*; et al.

Physical Review C, 83(6), p.064903_1 - 064903_29, 2011/06

https://doi.org/10.1103/PhysRevC.83.064903

Transverse momentum distributions and yields for , and in collisions at = 200 and 62.4 GeV at midrapidity are measured by the PHENIX experiment at the RHIC. We present the inverse slope parameter, mean transverse momentum, and yield per unit rapidity at each energy, and compare them to other measurements at different collisions. We also present the scaling properties such as and scaling and discuss the mechanism of the particle production in collisions. The measured spectra are compared to next-to-leading order perturbative QCD calculations.