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Journal Articles

Mutation analysis of high ethyl caproate producing sake yeasts generated by ion beam breeding

Masubuchi, Takashi*; Ueda, Ryoshiro; Kamiyama, Osamu*; Ikenaga, Hiroshi*; Sato, Katsuya; Narumi, Issey*

JAEA-Review 2013-059, JAEA Takasaki Annual Report 2012, P. 117, 2014/03

Journal Articles

Ion beam breeding of "Sake Yeast"

Masubuchi, Takashi*; Sato, Katsuya; Narumi, Issey*; Kamiyama, Osamu*

Bio Industry, 30(7), p.65 - 71, 2013/07

Sake is a useful tool for promoting local industry, but sake consumption has been decreasing year by year. In Gunma prefecture, the Gunma industrial technology center has playing a central role in breeding original sake yeast. Furthermore, for quickly corresponding with the needs from market, JAEA have tried breeding of sake yeast by ion beam breeding technology for development of distinctive sake yeast and its practical use while closely coordinating with the Gunma industrial technology center. As a result, the high ethyl caproate producing novel sake yeast were obtained.

Journal Articles

Identification of mutation sites in high ethyl caproate producing sake yeasts generated by ion beam breeding

Masubuchi, Takashi*; Takashima, Chisato*; Kamiyama, Osamu*; Ikenaga, Hiroshi*; Sato, Katsuya; Narumi, Issei

JAEA-Review 2012-046, JAEA Takasaki Annual Report 2011, P. 110, 2013/01

Journal Articles

Ion beam breeding of "sake yeast" and test brewing, 2

Masubuchi, Takashi*; Kamiyama, Osamu*; Sato, Katsuya; Hase, Yoshihiro; Narumi, Issei

JAEA-Review 2011-043, JAEA Takasaki Annual Report 2010, P. 113, 2012/01

Journal Articles

Ion beam breeding of "Sake Yeast" and test brewing

Masubuchi, Takashi*; Kamiyama, Osamu*; Sato, Katsuya; Hase, Yoshihiro; Narumi, Issei

JAEA-Review 2010-065, JAEA Takasaki Annual Report 2009, P. 82, 2011/01

no abstracts in English

Journal Articles

Ion beam breeding and test brewing of novel sake yeast

Masubuchi, Takashi*; Kamiyama, Osamu*; Sato, Katsuya; Hase, Yoshihiro; Narumi, Issei

Hoshasen To Sangyo, (128), p.43 - 47, 2010/12

no abstracts in English

Journal Articles

Ion beam breeding of "Sake Yeast"

Masubuchi, Takashi*; Kamiyama, Osamu*; Sato, Katsuya; Hase, Yoshihiro; Narumi, Issei

JAEA-Review 2009-041, JAEA Takasaki Annual Report 2008, P. 84, 2009/12

no abstracts in English

Oral presentation

Development of core damage evaluation technology (level 2 PSA) for fast reactors, 1; Summary and scope

Niwa, Hajime; Kurisaka, Kenichi; Sato, Ikken; Tobita, Yoshiharu; Kamiyama, Kenji; Yamano, Hidemasa; Miyahara, Shinya; Ohno, Shuji; Seino, Hiroshi; Ishikawa, Hiroyasu; et al.

no journal, , 

In order to develop the core damage evaluation technology (level 2 PSA) for sodium-cooled fast reactors, we develop the new analysis codes of post accident material relocation phase and of ex-vessel events, and we develop the technical bases that is necessary for level 2 PSA. In this presentation, summary and scope of the entire study is introduced as a part of the 4 series presentations.

Oral presentation

Development of core damage evaluation technology (level 2 PSA) for fast reactors, 5; Progress of R&D in FY2007

Nakai, Ryodai; Kurisaka, Kenichi; Sato, Ikken; Tobita, Yoshiharu; Kamiyama, Kenji; Yamano, Hidemasa; Miyahara, Shinya; Ohno, Shuji; Seino, Hiroshi; Ishikawa, Hiroyasu; et al.

no journal, , 

To develop a core damage evaluation technology (level-2 PSA) in sodium-cooled fast reactors, a new analysis method is developed for core-material relocation phase and internal containment vessel event. This study also develop technical basis necessary for the level-2 PSA.

Oral presentation

Ion beam breeding of Sake yeast

Masubuchi, Takashi*; Kamiyama, Osamu*; Sato, Katsuya; Hase, Yoshihiro; Narumi, Issei

no journal, , 

no abstracts in English

Oral presentation

Pulse neutron imaging experiments in the first year of J-PARC

Kai, Tetsuya; Harada, Masahide; Oikawa, Kenichi; Sakai, Kenji; Maekawa, Fujio; Oi, Motoki; Shinohara, Takenao; Aizawa, Kazuya; Takata, Shinichi; Arai, Masatoshi; et al.

no journal, , 

Development of imaging techniques with pulse neutrons were started using the NeutrOn Beam-line for Observation and Research Use (NOBORU) of J-PARC at December 2008. The results in the first three months, future research plans and discussions on a new imaging facility in J-PARC are shown.

Oral presentation

Breeding of a new sake yeast using ion beams and its test brewing

Masubuchi, Takashi*; Kamiyama, Osamu*; Sato, Katsuya; Hase, Yoshihiro; Narumi, Issei

no journal, , 

no abstracts in English

Oral presentation

Breeding of a new sake yeast using ion beams and its test brewing, 2

Masubuchi, Takashi*; Kamiyama, Osamu*; Sato, Katsuya; Hase, Yoshihiro; Narumi, Issei

no journal, , 

no abstracts in English

Oral presentation

Breeding of a new sake yeast using ion beams and its test brewing, 3

Masubuchi, Takashi*; Kamiyama, Osamu*; Sato, Katsuya; Hase, Yoshihiro; Tejima, Kohei; Narumi, Issei; Onodera, Takefumi

no journal, , 

no abstracts in English

Oral presentation

Gene expression analysis of sake yeast generated by ion beam breeding technology

Tomizawa, Yuki*; Hayashi, Hidenori*; Masubuchi, Takashi*; Kamiyama, Osamu*; Sato, Katsuya; Narumi, Issei; Ikenaga, Hiroshi*

no journal, , 

no abstracts in English

Oral presentation

Identification of mutation sites in high ethyl caproate producing sake yeats generated by ion beam breeding

Masubuchi, Takashi*; Kamiyama, Osamu*; Takashima, Chisato*; Ikenaga, Hiroshi*; Sato, Katsuya; Narumi, Issei

no journal, , 

no abstracts in English

Oral presentation

Mutation analysis of high ethyl caproate producing sake yeasts generated by ion beam breeding

Sato, Katsuya; Ueda, Ryoshiro; Masubuchi, Takashi*; Kamiyama, Osamu*; Hayashi, Hidenori*; Ikenaga, Hiroshi*; Narumi, Issey*; Tanaka, Atsushi

no journal, , 

We have previously obtained high ethyl caproate producing Sake yeasts "No.227" and "No.1333" by ion beam breeding. The fatty acid synthase genes (${it FAS1}$ and ${it FAS2}$) and the fatty acid esterase genes (${it EHT1}$ and ${it EEB1}$) might be involved in the higher production of ethyl caproate in the mutant strains. In this study, to identify the mutation sites of the mutant strains, each gene locus was sequenced. For the ${it EHT1}$, ${it EEB1}$ and ${it FAS1}$ loci, all the mutations found in mutant strains are nonsense mutations, suggesting that these mutations did not involved in the higher production of ethyl caproate. On the other hand, strains No.227 and No.1333 carried G/A and A/A mutations in ${it FAS2}$ gene at nucleotide position 3,748 as heterozygous and homozygous states, respectively. This result strongly suggested that the higher production of ethyl caproate in strains No.227 and No.1333 is attributed to a dominant mutation in ${it FAS2}$ gene.

Oral presentation

Good flavor Japanese sake generated by ion beam breeding technology

Sato, Katsuya; Masubuchi, Takashi*; Kamiyama, Osamu*; Ueda, Ryoshiro; Hayashi, Hidenori*; Ikenaga, Hiroshi*; Narumi, Issey*

no journal, , 

Sake is a useful tool for promoting local industry, but sake consumption has been decreasing year by year. In Gunma prefecture, the Gunma industrial technology center has playing a central role in breeding original sake yeast. Furthermore, for quickly corresponding with the needs from market, JAEA have tried breeding of sake yeast by ion beam breeding technology for development of distinctive sake yeast and its practical use while closely coordinating with the Gunma industrial technology center. As a result, the high ethyl caproate producing novel sake yeast were obtained.

Oral presentation

Mutation analysis of high ethyl caproate producing sake yeasts generated by ion beam breeding

Masubuchi, Takashi*; Kamiyama, Osamu*; Ueda, Ryoshiro; Ikenaga, Hiroshi*; Hayashi, Hidenori*; Sato, Katsuya; Narumi, Issey*

no journal, , 

We have previously obtained high ethyl caproate producing Sake yeasts "No.227" and "No.1333" by ion beam breeding. The fatty acid synthase genes (${it FAS1}$ and ${it FAS2}$) and the fatty acid esterase genes (${it EHT1}$ and ${it EEB1}$) might be involved in the higher production of ethyl caproate in the mutant strains. In this study, to identify the mutation sites of the mutant strains, each gene locus was sequenced. For the ${it EHT1}$, ${it EEB1}$ and ${it FAS1}$ loci, all the mutations found in mutant strains are nonsense mutations, suggesting that these mutations did not involved in the higher production of ethyl caproate. On the other hand, strains No.227 and No.1333 carried G/A and A/A mutations in ${it FAS2}$ gene at nucleotide position 3,748 as heterozygous and homozygous states, respectively. This result strongly suggested that the higher production of ethyl caproate in strains No.227 and No.1333 is attributed to a dominant mutation in ${it FAS2}$ gene.

Oral presentation

Analysis of sake yeast transcription system of galactose assimilating genes

Ikenaga, Hiroshi*; Tomizawa, Yuki*; Hikokubo, Kazuya*; Hayashi, Hidenori*; Masubuchi, Takashi*; Kamiyama, Osamu*; Sato, Katsuya; Narumi, Issey*

no journal, , 

no abstracts in English

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