Ion beam breeding of "Sake Yeast"

Masubuchi, Takashi*; Sato, Katsuya; Narumi, Issey*; Kamiyama, Osamu*

Bio Industry, 30(7), p.65 - 71, 2013/07

Sake is a useful tool for promoting local industry, but sake consumption has been decreasing year by year. In Gunma prefecture, the Gunma industrial technology center has playing a central role in breeding original sake yeast. Furthermore, for quickly corresponding with the needs from market, JAEA have tried breeding of sake yeast by ion beam breeding technology for development of distinctive sake yeast and its practical use while closely coordinating with the Gunma industrial technology center. As a result, the high ethyl caproate producing novel sake yeast were obtained.

Ion beam breeding of "sake yeast" and test brewing, 2

Masubuchi, Takashi*; Kamiyama, Osamu*; Sato, Katsuya; Hase, Yoshihiro; Narumi, Issei

JAEA-Review 2011-043, JAEA Takasaki Annual Report 2010, P. 113, 2012/01

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2011-043.pdf

Ion beam breeding and test brewing of novel sake yeast

Masubuchi, Takashi*; Kamiyama, Osamu*; Sato, Katsuya; Hase, Yoshihiro; Narumi, Issei

Hoshasen To Sangyo, (128), p.43 - 47, 2010/12

http://ci.nii.ac.jp/naid/40017417223

no abstracts in English

Identification of mutation sites in high ethyl caproate producing sake yeats generated by ion beam breeding

Masubuchi, Takashi*; Kamiyama, Osamu*; Takashima, Chisato*; Ikenaga, Hiroshi*; Sato, Katsuya; Narumi, Issei

no journal, , 

no abstracts in English

Ion beam breeding of Sake yeast

Masubuchi, Takashi*; Kamiyama, Osamu*; Sato, Katsuya; Hase, Yoshihiro; Narumi, Issei

no journal, , 

no abstracts in English

Gene expression analysis of sake yeast generated by ion beam breeding technology

Tomizawa, Yuki*; Hayashi, Hidenori*; Masubuchi, Takashi*; Kamiyama, Osamu*; Sato, Katsuya; Narumi, Issei; Ikenaga, Hiroshi*

no journal, , 

no abstracts in English

Mutation analysis of high ethyl caproate producing sake yeasts generated by ion beam breeding

Sato, Katsuya; Ueda, Ryoshiro; Masubuchi, Takashi*; Kamiyama, Osamu*; Hayashi, Hidenori*; Ikenaga, Hiroshi*; Narumi, Issey*; Tanaka, Atsushi

no journal, , 

We have previously obtained high ethyl caproate producing Sake yeasts "No.227" and "No.1333" by ion beam breeding. The fatty acid synthase genes ( and ) and the fatty acid esterase genes ( and ) might be involved in the higher production of ethyl caproate in the mutant strains. In this study, to identify the mutation sites of the mutant strains, each gene locus was sequenced. For the , and loci, all the mutations found in mutant strains are nonsense mutations, suggesting that these mutations did not involved in the higher production of ethyl caproate. On the other hand, strains No.227 and No.1333 carried G/A and A/A mutations in gene at nucleotide position 3,748 as heterozygous and homozygous states, respectively. This result strongly suggested that the higher production of ethyl caproate in strains No.227 and No.1333 is attributed to a dominant mutation in gene.

Mutation analysis of high ethyl caproate producing sake yeasts generated by ion beam breeding

Masubuchi, Takashi*; Kamiyama, Osamu*; Ueda, Ryoshiro; Ikenaga, Hiroshi*; Hayashi, Hidenori*; Sato, Katsuya; Narumi, Issey*

no journal, , 

We have previously obtained high ethyl caproate producing Sake yeasts "No.227" and "No.1333" by ion beam breeding. The fatty acid synthase genes ( and ) and the fatty acid esterase genes ( and ) might be involved in the higher production of ethyl caproate in the mutant strains. In this study, to identify the mutation sites of the mutant strains, each gene locus was sequenced. For the , and loci, all the mutations found in mutant strains are nonsense mutations, suggesting that these mutations did not involved in the higher production of ethyl caproate. On the other hand, strains No.227 and No.1333 carried G/A and A/A mutations in gene at nucleotide position 3,748 as heterozygous and homozygous states, respectively. This result strongly suggested that the higher production of ethyl caproate in strains No.227 and No.1333 is attributed to a dominant mutation in gene.

Development of core damage evaluation technology (level 2 PSA) for fast reactors, 5; Progress of R&D in FY2007

Nakai, Ryodai; Kurisaka, Kenichi; Sato, Ikken; Tobita, Yoshiharu; Kamiyama, Kenji; Yamano, Hidemasa; Miyahara, Shinya; Ohno, Shuji; Seino, Hiroshi; Ishikawa, Hiroyasu; et al.

no journal, , 

To develop a core damage evaluation technology (level-2 PSA) in sodium-cooled fast reactors, a new analysis method is developed for core-material relocation phase and internal containment vessel event. This study also develop technical basis necessary for the level-2 PSA.

Breeding of a new sake yeast using ion beams and its test brewing, 3

Masubuchi, Takashi*; Kamiyama, Osamu*; Sato, Katsuya; Hase, Yoshihiro; Tejima, Kohei; Narumi, Issei; Onodera, Takefumi

no journal, , 

no abstracts in English