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Fujimoto, Hirofumi*; Higuchi, Mariko; Koike, Manabu*; Ode, Hirotaka*; Pinak, M.; Kotulic Bunta, J.*; Nemoto, Toshiyuki*; Sakudo, Takashi*; Honda, Naoko*; Maekawa, Hideaki*; et al.
Journal of Computational Chemistry, 33(3), p.239 - 246, 2012/01
Times Cited Count：28 Percentile：61.66(Chemistry, Multidisciplinary)Lysine acetylation is one of the most common protein post transcriptional modifications. The acetylation effects of lysine residues on Ku protein were examined herein applying several computer simulation techniques. Acetylation of the lysine residues did not reduce the affinity between Ku and its substrate, DNA, in spite of the fact that the substitution of lysine with glutamine (KQ mutant) reduced the affinity of Ku for DNA, or the substitution of lysine with arginine (KR mutant) did not reduce it, as previously reported in experimental studies. These results suggest that the effects of in vivo acetylation may be overestimated when the KQ mutant is employed in mimicry of the acetylated protein.
Saegusa, Hiromitsu; Takeuchi, Shinji; Maekawa, Keisuke; Osawa, Hideaki; Semba, Takeshi
Proceedings of 13th International Conference on Environmental Remediation and Radioactive Waste Management (ICEM 2010) (CD-ROM), p.133 - 140, 2010/10
Kawasuso, Atsuo; Arashima, Hironobu*; Maekawa, Masaki; Ito, Hideaki*; Kabutomori, Toshiki*
Journal of Alloys and Compounds, 486(1-2), p.278 - 283, 2009/06
Times Cited Count：9 Percentile：49.3(Chemistry, Physical)We have studied the degradation process of TiCrV hydrogen storage alloy alloy using positron annihilation spectroscopy. As the hydrogen storage capacity decreased with the number of pressure swing cycles, positron lifetime increased. The increase in positron lifetime is due to the volume expansion caused by hydride formation. After degassing at 500 
C, the hydrogen storage capacity recovered to the initial level. However, the positron lifetime was still longer than the initial level suggesting the survival of dislocations. The degradation of hydrogen storage capacity is probably caused by both hydride formation and the generation of dislocations.
Osawa, Hideaki; Ota, Kunio; Hama, Katsuhiro; Sawada, Atsushi; Takeuchi, Shinji; Amano, Kenji; Saegusa, Hiromitsu; Matsuoka, Toshiyuki; Miyamoto, Tetsuo; Toyoda, Gakuji; et al.
JAEA-Research 2008-085, 742 Pages, 2008/11
JAEA-Research-2008-085-1.pdf:26.22MBJAEA-Research-2008-085-2.pdf:16.64MBJAEA-Research-2008-085-3.pdf:28.27MB
This report shows the results the project for the establishment of comprehensive site characterization technology, entrusted from Natural Resources and Energy Agency, Ministry of Economy, Trade and Industry Natural Resources and Energy Agency in 2007.
Kawasuso, Atsuo; Arashima, Hironobu*; Maekawa, Masaki; Ito, Hideaki*; Kabutomori, Toshiki*
Materials Science Forum, 607, p.122 - 124, 2008/11
Using positron lifetime spectroscopy, we examined the evolution of defects in the Ti
Cr
V
alloy prepared by the arc-melting method during hydriding cycles. After one hydriding cycle dislocations were responsible for positron trapping. The dislocation-related lifetime showed no significant change with increasing the hydriding cycle suggesting that the dislocation density is well above the dynamic range of positron trapping rate. After 20 hydriding cycles, prolonged lifetime components (0.4-0.5 ns and 1.9-2 ns) were obtained. These lifetimes were nearly constant during the further hydriding cycles while their intensities increased. Vacancy defects were generated and slowly developed to microvoids during the hydriding cycles. After 200 hydriding cycles the rechargeable hydrogen capacity decreased to 90% relative to the initial amount. It is thus inferred that the reduction of rechargeable hydrogen capacity is partly caused by the formation of microvoids and dislocations.
Yamauchi, Emiko*; Watanabe, Ritsuko; Oikawa, Miyoko*; Fujimoto, Hirofumi*; Yamada, Akinori*; Saito, Kimiaki; Murakami, Masahiro*; Hashido, Kazuo*; Tsuchida, Kozo*; Takada, Naoko*; et al.
Journal of Insect Biotechnology and Sericology, 77(1), p.17 - 24, 2008/02
The frequency of single strand breaks (SSBs) occurring on both strands of the pBR322 plasmid DNA region flanked by a pair of primers used for polymerase chain reaction (PCR) amplifications was determined after irradiation with 
Cs 
-rays. We refined that real time PCR is suitable for the detection and quantitative analysis of SSBs caused by -ray irradiation. The utility of this approach was also supported by the comparison of the practical experimental data with the Monte Carlo simulation. The potential application of this PCR method for the detection of genomic DNA damage was also confirmed.
Umeda, Koji; Oi, Takao; Osawa, Hideaki; Oyama, Takuya; Oda, Chie; Kamei, Gento; Kuji, Masayoshi*; Kurosawa, Hideki; Kobayashi, Yasushi; Sasaki, Yasuo; et al.
JAEA-Review 2007-050, 82 Pages, 2007/12
JAEA-Review-2007-050.pdf:28.56MB
This report shows the annual report which shows the summarized results and topic outline of each project on geological disposal technology in the fiscal year of 2006.
Fujimoto, Hirofumi*; Pinak, M.; Nemoto, Toshiyuki*; O'Neill, P.*; Kume, Etsuo; Saito, Kimiaki; Maekawa, Hideaki*
Journal of Computational Chemistry, 26(8), p.788 - 798, 2005/06
Times Cited Count：23 Percentile：57.59(Chemistry, Multidisciplinary)Clustered DNA damage sites induced by ionizing radiation have been suggested to have serious consequences to organisms. In this study, approaches based on molecular dynamics (MD) simulation have been applied to examine conformational changes and energetic properties of DNA molecules containing clustered damage sites consisting of 2 lesioned sites, 8-oxoG and AP site. After 1 nanosecond of MD simulation, one of the 6 DNA molecules containing a clustered damage site develop specific characteristic features: sharp bending at the lesioned site and weakening or complete loss of electrostatic interaction energy between 8-oxoG and bases locating on the complementary strand. From these results it is suggested that these changes would make it difficult for the repair enzyme to bind to the lesions within the clustered damage site and thereby result in a reduction of its repair capacity.
Kasugai, Yoshimi; Takada, Hiroshi; Nakashima, Hiroshi; Sakata, Hideaki*; Maekawa, Fujio; Ikeda, Yujiro; Kawai, Masayoshi*; Ino, Takashi*; Jerde, E.*; Glasgow, D.*; et al.
JAERI-Conf 2001-002, p.955 - 963, 2001/03
no abstracts in English
Kawasuso, Atsuo; Maekawa, Masaki; Arashima, Hironobu*; Ito, Hideaki*; Kabutomori, Toshiki*
no journal, ,
no abstracts in English
-ray analysis on J-PARC NOBORUMatsue, Hideaki; Kasugai, Yoshimi; Harada, Masahide; Maekawa, Fujio; Kubo, Kenya*; Saito, Tsutomu*
no journal, ,
no abstracts in English
Iwatsuki, Teruki; Sato, Toshinori; Takeuchi, Ryuji; Onoe, Hironori; Onuki, Kenji; Saegusa, Hiromitsu; Hasegawa, Takashi; Kuroiwa, Hiroshi; Tsuyuguchi, Koji; Ikeda, Koki; et al.
no journal, ,
Closure experiment of gallery of MIU is planned to understand the recovery process of geological environments after the closure. Flooding test gallery and monitoring boreholes are designed at 500 m access gallery to observe recovery of water pressure, chemistry around cement and burial materials, rock stress. Evaluation of numerical analysis methods on hydraulic, chemical and mechanical change will be conducted based on the results of upcoming closure experiment.
Maekawa, Keisuke; Suzuki, Hideaki*; Sakai, Yuichi; Nakama, Shigeo; Sugita, Yutaka
no journal, ,
no abstracts in English
Kugo, Teruhiko; Hamada, Jumpei; Mineo, Hideaki; Okubo, Nariaki; Takano, Masahide; Matsumura, Tatsuro; Watanabe, Masayuki; Iwamoto, Osamu; Morita, Yasuji; Maekawa, Fujio
no journal, ,
The status of development of human resources in JAEA for research and development to support nuclear energy utilization is reported.
