Separation/removal of steel surface coating film by laser cleaning

Yamane, Ikumi; Takahashi, Nobuo; Sawayama, Kengo; Nishiwaki, Hiroki; Matsumoto, Takashi; Ogawa, Jumpei; Nomura, Mitsuo; Arima, Tatsumi*

JAEA-Technology 2021-038, 18 Pages, 2022/02

JAEA-Technology-2021-038.pdf:1.61MB

We have dismantled uranium enrichment facilities in Ningyo-toge Environmental Engineering Center since their operation finished in 2001, and the total amount of metallic wastes is estimated to be about 130 thousand tons. Eighty percent of them can be disposed as nonradioactive waste (NR), but there are some steel parts possibly uranium-contaminated. We need removing painted surface of such steels and radiologically surveying to dispose them as NRs. Though painted surfaces have been conventionally removed through hand working with grinders, this manual work requires installation of green house, protective clothing, and full-face mask, in order to prevent dispersion and inhalation of airborne dusts. We desire further developments of surface cleaning techniques to reduce time, cost, workload, and secondary waste generation caused by excessive grinding. Therefore, in this study, we focused on the laser cleaning technology used for the separation and removal of paint films at construction sites. In order to improve the coating separation and removal technology for NR objects, we evaluated the coating separation and removal performance of NR steel surface by laser cleaning system, observed the coating scattering behavior by high-speed camera and investigated the coating recovery method, evaluated the laser separation and removal performance of steel surface powder, and thermodynamically evaluated the uranium compounds on steel surface. We additionally evaluated the feasibility of laser cleaning techniques in our works basing on these results, and discussed future work plans for further developments of laser cleaning techniques.

Large-scale identification and characterization of alternative splicing variants of human gene transcripts using 56 419 completely sequenced and manually annotated full-length cDNAs

Takeda, Junichi*; Suzuki, Yutaka*; Nakao, Mitsuteru*; Barrero, R. A.*; Koyanagi, Kanako*; Jin, L.*; Motono, Chie*; Hata, Hiroko*; Isogai, Takao*; Nagai, Keiichi*; et al.

Nucleic Acids Research, 34(14), p.3917 - 3928, 2006/00

https://doi.org/10.1093/nar/gkl507

We report the first genome-wide identification and characterization of alternative splicing in human gene transcripts based on analysis of the full-length cDNAs. Applying both manual and computational analyses for 56 419 completely sequenced and precisely annotated full-length cDNAs selected for the H-Invitational human transcriptome annotation meetings, we identified 6877 alternative splicing genes with 18 297 different alternative splicing variants. A total of 37 670 exons were involved in these alternative splicing events. The encoded protein sequences were affected in 6005 of the 6877 genes. Notably, alternative splicing affected protein motifs in 3015 genes, subcellular localizations in 2982 genes and transmembrane domains in 1348 genes. Genome-wide annotations of alternative splicing, relying on full-length cDNAs, should lay firm groundwork for exploring in detail the diversification of protein function which is mediated by the alternative splicing variants.