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論文

Development of new ${it Chrysanthemum Morifolium}$ pink mutants through ion beam irradiation

Zaiton, A.*; Affrida, A. H.*; Shakinah, S.*; Nurul Hidayah, M.*; 長谷 純宏; 大野 豊

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 108, 2015/03

Developing new varieties of chrysanthemums is one of the emphases in Malaysian floriculture research as chrysanthemum is recognized as among the most important cut flower export for Malaysia. A combination of ion beam irradiation and ${it in vitro}$ organ culture techniques is very efficient in generating new ornamental varieties with novel traits and wider mutation spectrum. Therefore, in this project, ray floret cultures of pink chrysanthemum variety were irradiated at various doses with 320 MeV $$^{12}$$C$$^{6+}$$ ions, regenerated into plantlets and screened for morphological variations with an ultimate aim to produce mutants with useful horticultural traits. A series of screening was carried out at Nuclear Malaysia's glasshouse and MARDI Cameron Highlands. The mutants were selected and characterized morphologically. Based on these screenings, three mutants were identified based on their distinct flower and/or plant characteristics.

論文

Generating new chrysanthemum plant varieties using ion beams

Zaiton, A.*; Affrida, A. H.*; Shakinah, S.*; Nurul Hidayah, M.*; 野澤 樹*; 鳴海 一成*; 長谷 純宏; 大野 豊

JAEA-Review 2013-059, JAEA Takasaki Annual Report 2012, P. 104, 2014/03

Chrysanthemum is among the most important cut flowers in Malaysia especially for export market. The main problem for chrysanthemum in Malaysia is the lack of new varieties to meet the ever-changing customer demands. To generate new chrysanthemum plant varieties using ion beams, this report discusses irradiation experiments for nodal cultures of chrysanthemum Pink variety. ${it In vitro}$ stems were cut into single nodes (0.5 cm in length) and cultured approximately 5 days before irradiation. Data on the survival of cultures, regenerated shoot height and number of leaves were taken 8 weeks after irradiation. The results suggested that the sensitivity of nodes to ion beam irradiation is similar to that of their ray floret counterparts examined last year.

口頭

Achievement of nuclear Malaysia - JAEA bilateral project on generating new ornamental plant varieties using ion beams

Zaiton, A.*; Affrida, A. H.*; Shakinah, S.*; Nurul Hidayah, M.*; 野澤 樹*; 鳴海 一成*; 長谷 純宏; 大野 豊

no journal, , 

Malaysian Nuclear Agency and Japan Atomic Energy Agency have jointly involved in the Research Cooperation in the Field of Radiation Processing since 1987. Under this cooperation, the five-year program on "Generating New Ornamental Plant Varieties using Ion Beams" was implemented from 2007 to 2012. ${it Chrysanthemum morifolium}$ was chosen for this project as it is one of the most important ornamentals in Malaysia especially for cut flower export. The main objective was to develop new varieties with useful traits through a combination of ion beam irradiation and in vitro organ culture techniques. At present, 5 stable mutants of Reagan Red variety with distinct flower characters and two potential mutants from the Pink variety have been identified. Among the mutants, a Reagan Red mutant (irradiated by carbon ions at 2 Gy) with attractive features (purplish red and daisy-eyed double ray floret) is named TIARA Red.

口頭

New chrysanthemum mutant varieties developed through ion beam irradiation technology

Zaiton, A.*; Affrida, A. H.*; Shakinah, S.*; Nurul Hidayah, M.*; 野澤 樹*; 鳴海 一成*; 長谷 純宏; 大野 豊

no journal, , 

New chrysanthemum mutants were successfully developed from a collaborative project entitled "Generating New Ornamental Plant Varieties using Ion Beams" between Nuclear Malaysia and JAEA. These mutants, of Reagan Red and Pink varieties, were generated through a combination of ion beam irradiation and in vitro organ culture techniques. Chrysanthemum cultures were irradiated at different doses of carbon ions. The irradiated explants were grown to full plantlets and sub-cultured for four vegetative cycles before being planted in the glasshouses for morphological screenings. Eight new mutants were selected based on the uniqueness of their features.

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