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Okamura, Masachika*; Hase, Yoshihiro; Furusawa, Yoshiya*; Tanaka, Atsushi
Euphytica, 202(3), p.333 - 343, 2015/04
Times Cited Count：15 Percentile：63.88(Agronomy)We investigated the effect of tissue source selection in combination with ion beam irradiation on generating flower-color mutants in Chrysanthemum morifolium. Petal and leaf tissues of the chrysanthemum cultivar YoMystery (purple flowers) were used. Somaclones regenerated from irradiated tissues were analyzed for stem length and flower color mutation. Three Gy of argon ion beams was regarded as an appropriate dose for irradiation because the resulting color mutants maintained an adequate stem length suitable for commercial use. Statistical analysis of 7,258 individuals from three separate experiments revealed that (1) somaclones from petal tissue had a higher mutation frequency (2.91%) than leaf somaclones (2.01%); (2) the effect of tissue source on the frequency of flower color mutations was significantly enhanced when combined with ion-beam irradiation in that the mutation frequency of ion-beam irradiated petal clones was 6.47%, whereas that of ion-beam irradiated leaf clones was 3.89%; and (3) the spectrum of carotenoid color mutations was remarkably increased by ion-beam irradiation in that both leaf and petal ion-beam irradiated clones had brown, red and yellow flowers in contrast to the somaclones that only produced yellow flowers. These results suggest that intentional or directed induction of flower color mutants with practical value is possible by combining tissue source selection with ion beam irradiation.
Okamura, Masachika*; Nakayama, Masayoshi*; Umemoto, Naoyuki*; Cano, E. A.*; Hase, Yoshihiro; Nishizaki, Yuzo*; Sasaki, Nobuhiro*; Ozeki, Yoshihiro*
Euphytica, 191(1), p.45 - 56, 2013/05
Times Cited Count：28 Percentile：80.53(Agronomy)A few carnation cultivars are known to display a peculiar dusky color supposedly caused by anthocyanic vacuolar inclusions (AVIs). The hereditary pattern suggests that the peculiar color is controlled by a single recessive factor tightly linked with existence of AVIs containing non-acylated anthocyanins. To diversify the peculiar color carnation, we produced a bluish purple line displaying a highly novel metallic appearance by crossbreeding. By subjecting the line to ion-beam irradiation, we generated metallic reddish purple, metallic crimson and metallic red lines. All four metallic lines did not have transcripts for anthocyanin malyltransferase. In contrast to the dusky color types, metallic lines have highly condensed AVIs and water-clear vacuolar sap in the petal adaxial epidermal cells. We demonstrated that (1) a factor generating the AVIs is inactivated anthocyanin malyltransferase gene, (2) AVIs in water-clear vacuolar sap in the adaxial epidermal cells generate the novel metallic appearance, and (3) ion beam breeding is a useful tool for increasing metallic colors by changing anthocyanin structure and the level of AVIs.
Okamura, Masachika*; Hase, Yoshihiro; Onishi, Noboru*; Narumi, Issei; Tanaka, Atsushi
JAEA-Review 2011-043, JAEA Takasaki Annual Report 2010, P. 105, 2012/01
Hase, Yoshihiro; Okamura, Masachika*; Takeshita, Daigaku*; Narumi, Issei; Tanaka, Atsushi
Plant Biotechnology, 27(1), p.99 - 103, 2010/03
Times Cited Count：26 Percentile：60.02(Biotechnology & Applied Microbiology)Hase, Yoshihiro; Okamura, Masachika*; Takeshita, Daigaku*; Narumi, Issei; Tanaka, Atsushi
JAEA-Review 2009-041, JAEA Takasaki Annual Report 2008, P. 66, 2009/12
Okamura, Masachika*; Takeshita, Daigaku*; Hase, Yoshihiro; Narumi, Issei; Tanaka, Atsushi
JAEA-Review 2009-041, JAEA Takasaki Annual Report 2008, P. 69, 2009/12
Okamura, Masachika*; Shimizu, Akira*; Onishi, Noboru*; Hase, Yoshihiro; Yoshihara, Ryohei; Narumi, Issei
JAEA-Review 2008-055, JAEA Takasaki Annual Report 2007, P. 62, 2008/11
no abstracts in English
Okamura, Masachika*; Shimizu, Akira*; Watanabe, Satomi*; Hase, Yoshihiro; Narumi, Issei; Tanaka, Atsushi
JAEA-Review 2007-060, JAEA Takasaki Annual Report 2006, P. 82, 2008/03
no abstracts in English
Okamura, Masachika*; Momose, Masayuki*; Watanabe, Satomi*; Shimizu, Akira*; Hase, Yoshihiro; Yokota, Yuichiro; Tanaka, Atsushi
JAEA-Review 2006-042, JAEA Takasaki Annual Report 2005, P. 82, 2007/02
no abstracts in English
Okamura, Masachika*; Tanaka, Atsushi; Momose, Masaki*; Umemoto, Naoyuki*; Silva, J.*; Toguri, Toshihiro*
Floriculture, Ornamental and Plant Biotechnology; Advances and Topical Issues, Vol.1, p.619 - 628, 2006/00
no abstracts in English
Okamura, Masachika*; Yasuno, Noriko*; Otsuka, Masako*; Tanaka, Atsushi; Shikazono, Naoya; Hase, Yoshihiro
Nuclear Instruments and Methods in Physics Research B, 206, p.574 - 578, 2003/05
Times Cited Count：91 Percentile：97.71(Instruments & Instrumentation)Resent studies indicate that the ion beams have higher mutation frequency than low-LET radiations in plants but the difference in mutation spectrum still remains to be characterized. We investigated the efficiency of ion-beam irradiation combined with tissue culture in obtaining floral mutants. Leaves collected from carnation plants, cultivar Vital (cherry pink flowers with frilly petals), were irradiated with carbon ions or X-rays. They were cultured till the shoots regenerated. Sixteen mutants were obtained from 705 regenerated plants by carbon-ion irradiation. Those mutants were rich in variety, i.e., pink, dark pink, light pink, salmon, red, complex- and striped-color, and round and Dianthus-type petals were obtained. In contrast, 7 mutants obtained from 556 regenerated plants by X-rays were only pink, light pink and red. These results indicate that the ion beams could induce wider variety of flower-color and shape mutant than X-rays, and also indicate that the combined method of ion-beam irradiation with tissue culture is useful to obtain commercial varieties in a short time.
Okamura, Masachika*; Momose, Masaki*; Watanabe, Satomi*; Tanaka, Atsushi; Hase, Yoshihiro
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Okamura, Masachika*; Watanabe, Satomi*; Tanaka, Atsushi; Hase, Yoshihiro
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Okamura, Masachika*; Momose, Masaki*; Umemoto, Naoyuki*; Toguri, Toshihiro*; Tanaka, Atsushi; Hase, Yoshihiro; Yamaguchi, Hiroyasu*; Morishita, Toshikazu*
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Okamura, Masachika*; Watanabe, Satomi*; Shimizu, Akira*; Watanabe, Maki*; Hase, Yoshihiro; Yokota, Yuichiro; Narumi, Issei; Tanaka, Atsushi
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Okamura, Masachika*; Umemoto, Naoyuki*; Hase, Yoshihiro; Yoshihara, Ryohei; Narumi, Issei
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Hase, Yoshihiro; Narumi, Issei; Tanaka, Atsushi; Okamura, Masachika*; Takeshita, Daigaku*
no journal, ,
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Okamura, Masachika*; Takeshita, Daigaku*; Hase, Yoshihiro; Nozawa, Shigeki; Narumi, Issei; Tanaka, Atsushi
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Hase, Yoshihiro; Okamura, Masachika*; Takeshita, Daigaku*; Narumi, Issei; Tanaka, Atsushi
no journal, ,
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Okamura, Masachika*; Hase, Yoshihiro; Umemoto, Naoyuki*; Nakayama, Masayoshi*; Tanikawa, Natsu*; Narumi, Issei; Tanaka, Atsushi
no journal, ,
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