Effect of nitrate on nodulation and nitrogen fixation of soybean

Oyama, Takuji; Fujikake, Hiroyuki*; Yashima, Hiroyuki*; Tanabata, Sayuri*; Ishikawa, Shinji*; Sato, Takashi*; Nishiwaki, Toshikazu*; Otake, Norikuni*; Sueyoshi, Kuni*; Ishii, Satomi; et al.

Soybean Physiology and Biochemistry, p.333 - 364, 2011/11

https://doi.org/10.5772/17992

no abstracts in English

The Production of N-labeled nitrogen gas tracer and the imaging of nitrogen fixation in soybean nodules

Ishii, Satomi; Suzui, Nobuo; Ito, Sayuri; Ishioka, Noriko; Kawachi, Naoki; Matsuhashi, Shimpei; Otake, Norikuni*; Oyama, Takuji*; Fujimaki, Shu

JAEA-Review 2009-041, JAEA Takasaki Annual Report 2008, P. 106, 2009/12

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2009-041.pdf

The Production of N-labeled nitrogen gas for imaging of nitrogen fixation by soybean nodule

Ishii, Satomi; Suzui, Nobuo; Ishioka, Noriko; Kawachi, Naoki; Matsuhashi, Shimpei; Otake, Norikuni*; Oyama, Takuji*; Fujimaki, Shu

JAEA-Review 2008-055, JAEA Takasaki Annual Report 2007, P. 111, 2008/11

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2008-055.pdf

A Non-invasive visualizing technique for observing the symbiotic nitrogen fixation using N

Fujimaki, Shu; Ishii, Satomi; Suzui, Nobuo; Ito, Sayuri; Kawachi, Naoki; Otake, Norikuni*; Oyama, Takuji*; Ishioka, Noriko

no journal, , 

The positron-emitting tracer imaging system (PETIS) is one of the non-invasive imaging tools for living organisms. PETIS is specialized for plant nutrition with very compact detection heads which can be installed in a growth chamber. We have been developing the total methods of PETIS, including production of various positron-emitting radiotracers such as Cd, and many studies in plant nutrition have been done using PETIS in this decade. Here, we employed N for the study of symbiotic nitrogen fixation and successfully developed a rapid method to produce and purify N-labeled nitrogen gas. The gaseous tracer was fed as a mixture of N, O and He (10:20:70) to the underground part of intact nodulated soybean plants. As a result, serial images of distribution of the fixed N were obtained, and the fixation rates were estimated 0.170.10 mol N h plant (mean SD, n=6) from the data.

Noninvasive imaging of nitrogen fixation in soybean nodules

Ishii, Satomi; Suzui, Nobuo; Ito, Sayuri; Kawachi, Naoki; Ishioka, Noriko; Otake, Norikuni*; Oyama, Takuji*; Fujimaki, Shu

no journal, , 

no abstracts in English

Quantitative analysis of transport of fixed nitrogen from soybean nodule using N as a tracer

Hung, N. V. P.; Watanabe, Shiori*; Ishikawa, Shinji*; Otake, Norikuni*; Sueyoshi, Kuni*; Ishii, Satomi; Fujimaki, Shu; Oyama, Takuji*; Yashima, Hiroyuki*

no journal, , 

Biological Nitrogen Fixation (BNF) plays an important role in the plant life especial in legume species. Through symbiotic fixation process, legume plants can use atmospheric nitrogen as nutritional source for their growth and development. Soybean plants have an ability to fix dinitrogen (N) in the atmosphere in root nodules and absorb nitrogen either from fertilizer or soil N. Soybean needs a large amount of N to synthesize seed storage protein especial in stage of pod filling. Many studies have been carried out in the fields of BNF, but up to now the accurate measurement of transport rate of fixed N has not determined yet. Until now, there are many methods to investigate nitrogen fixation and transportation in leguminous plants. This study used N isotope as a tracer to quantify nitrogen fixation and the transport rate of fixed N to various parts of shoots and roots in nodulated soybean plants during relatively short time from 1 h to 8h.

Imaging of nitrogen fixation in nodulated legume by using N

Ishii, Satomi; Otake, Norikuni*; Ishioka, Noriko; Suzui, Nobuo; Fujimaki, Shu; Matsuhashi, Shimpei; Oyama, Takuji*

no journal, , 

no abstracts in English

Visualization of nitrogen fixation in an intact soybean plant with nodules by using positron-imaging method

Ishii, Satomi; Suzui, Nobuo; Ito, Sayuri; Ishioka, Noriko; Kawachi, Naoki; Otake, Norikuni*; Oyama, Takuji*; Fujimaki, Shu

no journal, , 

no abstracts in English

Analysis of the initial transport of fixed nitrogen and the effect of O partial pressure on nitrogen fixation in soybean using nitrogen isotopes

Hung, N. V. P.; Watanabe, Shiori*; Ishikawa, Shinji*; Otake, Norikuni*; Sueyoshi, Kuni*; Sato, Takashi*; Ishii, Satomi; Suzui, Nobuo; Kawachi, Naoki; Yin, Y.-G.; et al.

no journal, , 

The Synthesis of N labeled nitrogen gas for imaging of nitrogen fixation by soybean nodule

Ishii, Satomi; Suzui, Nobuo; Ishioka, Noriko; Kawachi, Naoki; Matsuhashi, Shimpei; Fujimaki, Shu; Otake, Norikuni*; Oyama, Takuji*

no journal, , 

no abstracts in English