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(Hypocreales: Clavicipitaceae) mutants induced by ion beamsFitriana, Y.*; Shinohara, Shinobu*; Sato, Katsuya; Narumi, Issey*; Saito, Tsutomu*
Applied Entomology and Zoology, 50(1), p.123 - 129, 2015/02
Times Cited Count：0 Percentile：1.75(Chemistry, Physical)
following ionizing radiation-induced mutagenesisShinohara, Shinobu*; Fitriana, Y.*; Sato, Katsuya; Narumi, Issey*; Saito, Tsutomu*
FEMS Microbiology Letters, 349(1), p.54 - 60, 2013/12
Times Cited Count：11 Percentile：29.5(Microbiology)
Sn content in spent nuclear fuel sampleAsai, Shiho; Toshimitsu, Masaaki; Hanzawa, Yukiko; Suzuki, Hideya; Shinohara, Nobuo; Inagawa, Jun; Okumura, Keisuke; Hotoku, Shinobu; Kimura, Takaumi; Suzuki, Kensuke*; et al.
Journal of Nuclear Science and Technology, 50(6), p.556 - 562, 2013/06
Times Cited Count：11 Percentile：64.2(Nuclear Science & Technology)The Sn content in a spent nuclear fuel solution was determined by ICP-MS for its inventory estimation in high-level radioactive waste. An irradiated UO
fuel was used as a sample to evaluate the reliability of the methodology. Prior to the measurement, Sn was separated from Te, which causes major isobaric interference in the determination of Sn content, along with highly radioactive coexisting elements using an anion-exchange column. The absence of counts attributed to Te in the Sn-containing effluent indicates that Te was completely removed. After washing, Sn retained on the column was readily eluted with 1 M HNO
. The isotope ratios of Sn were successfully determined and showed good agreement with those obtained through ORIGEN2 calculations. The results reported in this paper are the first experimental values of Sn content in the spent nuclear fuel solution originating in spent nuclear fuel irradiated at a nuclear power plant in Japan.
Saito, Tsutomu*; Shinohara, Shinobu*; Sato, Katsuya; Narumi, Issei
JAEA-Review 2012-046, JAEA Takasaki Annual Report 2011, P. 109, 2013/01
Saito, Tsutomu*; Shinohara, Shinobu*; Sato, Katsuya; Narumi, Issei
JAEA-Review 2011-043, JAEA Takasaki Annual Report 2010, P. 112, 2012/01
Se and 
Cs in spent nuclear fuel for inventory estimation of high-level radioactive wastesAsai, Shiho; Hanzawa, Yukiko; Okumura, Keisuke; Shinohara, Nobuo; Inagawa, Jun; Hotoku, Shinobu; Suzuki, Kensuke*; Kaneko, Satoru*
Journal of Nuclear Science and Technology, 48(5), p.851 - 854, 2011/05
Times Cited Count：25 Percentile：86.49(Nuclear Science & Technology)
irradiationShinohara, Shinobu*; Narumi, Issei; Amano, Kae*; Tagami, Yosuke*; Saito, Tsutomu*
no journal, ,
no abstracts in English
Se and Cs in high-level radioactive wastesAsai, Shiho; Hanzawa, Yukiko; Okumura, Keisuke; Inagawa, Jun; Hotoku, Shinobu; Shinohara, Nobuo; Suzuki, Kensuke*; Kaneko, Satoru*
no journal, ,
Shinohara, Shinobu*; Sato, Katsuya; Narumi, Issei; Tagami, Yosuke*; Saito, Tsutomu*
no journal, ,
no abstracts in English
Saito, Tsutomu*; Shinohara, Shinobu*; Sato, Katsuya; Narumi, Issei
no journal, ,
no abstracts in English
Shinohara, Shinobu*; Sato, Katsuya; Narumi, Issei; Tagami, Yosuke*; Saito, Tsutomu*
no journal, ,
Entomopathogenic fungi, Isaria fumosorosea and Beauveria bassianai, are important agents to control insect pests, but the fungi are highly susceptible to conventional fungicides for plant diseases. The purpose of this study is to generate mutants tolerant to fungicides (benomyl and triflumizole) using carbon ion beams. In the I. fumosorosea mutants, minimum inhibitory concentrations (MICs) of benomyl and triflumizole increased more than 5 and 4 times, respectively, compared to the original strain. In B. bassiana mutants, MICs of benomyl increased more than 5 times. Two-step irradiation with rays and carbon beams was tested. MICs in mutants obtained by two-step irradiation were similar to those in mutants obtained by single irradiation with carbon beams. These results indicate that carbon beam irradiation is greatly useful for making fungicide-tolerant mutants of entomopathogenic fungi. Further studies will be needed to determine pathogenicity of the mutants against host insects.
Shinohara, Shinobu*; Sato, Katsuya; Narumi, Issei; Tagami, Yosuke*; Saito, Tsutomu*
no journal, ,
Entomopathogenic fungi, and 
, are important agents to control insect pests, but the fungi are highly susceptible to conventional fungicides for plant diseases. The purpose of this study is to generate mutants tolerant to fungicides (benomyl and triflumizole) using quantum beams ( rays and carbon ion beams). Eighteen fungicide-tolerant mutants were selected on the medium supplemented by fungicides (1,000 ppm for benomyl and 150 ppm for trifulmizole). In 5 mutants, fungicide tolerant level, crossing relation with other fungicide and pathogenicity against host insects were investigated. As a result, the mutants that have the minimum inhibitory concentration for benomyl of approximately 5 times were successfully obtained, and yet these were maintained the pathogenicity.
Saito, Tsutomu*; Shinohara, Shinobu*; Sato, Katsuya; Narumi, Issei
no journal, ,
no abstracts in English
Saito, Tsutomu*; Fitriana, Y.*; Shinohara, Shinobu*; Sato, Katsuya; Narumi, Issey*
no journal, ,
In order to identify the mutation sites of 
-tubulin in the benomyl-tolerant mutants, we determined the nucleotide sequences of -tubulin locus and compared with those of the wild-type strains. In , the -tubulin sequences were identical to that of the wild-type, suggesting that other mechanisms might be responsible for the benomyl tolerance. On the other hand, in , the mutation was identified to be an A:T to C:G transversion at position 924 in the -tubulin gene. This mutation causes an amino acid substitution at position 198 (Glu to Ala) in the -tubulin protein. This site might be a binding target of benomyl or adjacent to the center of benomyl interaction with the -tubulin. These results suggest that mechanisms of benomyl tolerance were different between 
and 
.
