Distinct structural requirements for interleukin-4 (IL-4) and IL-13 binding to the shared IL-13 receptor facilitate cellular tuning of cytokine responsiveness

Ito, Takachika*; Suzuki, Shoichi*; Kanaji, Sachiko*; Shiraishi, Hiroshi*; Ota, Shoichiro*; Arima, Kazuhiko*; Tanaka, Go*; Tamada, Taro; Honjo, Eijiro*; Garcia, K. C.*; et al.

Journal of Biological Chemistry, 284(36), p.24289 - 24296, 2009/09

https://doi.org/10.1074/jbc.M109.007286

Both IL-4 and IL-13 can bind to the shared receptor composed of the IL-4 receptor chain and the IL-13 receptor -1 chain (IL-13R1); however, the assembly mechanisms of these ligands to the receptor is different, enabling the principal functions of these ligands to be different. We have previously shown that the N-terminal Ig-like domain in IL-13R1, called the D1 domain, is the specific and critical binding unit for IL-13. However, it has still remained obscure which the amino acid has specific binding capacity to IL-13 and why the D1 domain acts as the binding site for IL-13, but not IL-4. To address these questions, in this study, we performed the mutational analyses for the D1 domain, combining the structural data to identify the amino acids critical for binding to IL-13. Mutations of Lys76, Lys77, or Ile78 in c' strand in which the crystal structure showed interact with IL-13 and those of Trp65 and Ala79 adjacent to the interacting site, resulted in significant impairment of IL-13 binding, demonstrating that these amino acids generate the binding site. Furthermore, mutations of Val35, Leu38, or Val42 at N-terminal -strand also resulted in loss of IL-13 binding, probably from decrease structural stability. None of the mutations employed here affected IL-4 binding. These results demonstrate that the hydrophobic patch composed of Lys76, Lys77, and Ile78 is the IL-13 recognition site and solidify our understanding that the differential requirements of the D1 domain in IL-13R1 allows the shared receptor to respond differentially to IL-4 and IL-13.

Neutron irradiation effects on fusion reactor materials

Shiraishi, K.

Oyo Butsuri, 55(3), p.202 - 209, 1986/00

http://joi.jlc.jst.go.jp/JST.Journalarchive/oubutsu1932/55.202

no abstracts in English

Effect of carbon on microstructure in Ti-modified type 316 stainless steels irradiated with helium ions

Suzuki, K.; Katano, Y.; Aruga, T.; Hamada, S.; Shiraishi, K.

Journal of Nuclear Materials, 133-134, p.585 - 589, 1985/00

https://doi.org/10.1016/0022-3115(85)90215-6

no abstracts in English

Effect of helium on microstructure in type 316 stainless steel

Shiraishi, K.; ;

Journal of Nuclear Materials, 119(2-3), p.268 - 277, 1985/00

https://doi.org/10.1016/0022-3115(83)90203-9

no abstracts in English

Research and Development of Austenitic Stainless Steels for Fusim Reactor

Shiraishi, K.; *; *; *; *; *; *; *; *; *; et al.

JAERI-M 84-189, 220 Pages, 1984/09

JAERI-M-84-189.pdf:14.7MB

no abstracts in English

Helium bubble behavior in long term aged 316 and 316+Ti steels irradiated with helium ions

Aruga, T.; Katano, Y.; Shiraishi, K.

Journal of Nuclear Materials, 122-123, p.1401 - 1405, 1984/00

no abstracts in English

Effect of external stress on the microstructural change during electron-irradiation in nickel

; Katano, Y.; Shiraishi, K.

Journal of Nuclear Science and Technology, 21(9), p.671 - 677, 1984/00

https://doi.org/10.3327/jnst.21.671

no abstracts in English

Measurement of mean projected range for Ar-ion and N-ion irradiated stainless steels

; Shiraishi, K.; *; Hamada, S.;

Nihon Genshiryoku Gakkai-Shi, 26(11), p.974 - 976, 1984/00

https://doi.org/10.3327/jaesj.26.974

no abstracts in English

A Technique for observing the cross sectional damage profiles in He-ion irradiated 316 stainless steel by transmission

Hamada, S.; ; Shiraishi, K.

Journal of Nuclear Materials, 114, p.338 - 340, 1983/00

https://doi.org/10.1016/0022-3115(83)90275-1

no abstracts in English

Measurement of mean projected range for He-ion irradiated Stainless Steel, 2

; Shiraishi, K.

Nihon Genshiryoku Gakkai-Shi, 25(4), p.52 - 54, 1983/00

no abstracts in English