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Journal Articles

Corrosion resistance and oxide film structure of stainless steels and Ni-based alloys under sulfuric decomposition gas at high temperature

Hirota, Noriaki; Takeda, Kiyoko*; Tachibana, Yukio; Masaki, Yasuhiro*

Zairyo To Kankyo, 70(3), p.68 - 76, 2021/03

https://doi.org/10.3323/jcorr.70.68

Corrosion resistance of stainless steels and Ni-based alloys were evaluated in a sulfuric acid decomposition gas at high temperature. The evaluation were carried out in an environment simulated in the sulfuric acid decomposition reaction vessel for thermochemical hydrogen production process (IS process). Their corrosion films were also analyzed for better understanding of the corrosion behavior. As a result, after 100 hour corrosion test, Ni-based alloy containing 2.4% Si showed good corrosion resistance. Ferritic stainless steel containing 3% Al (3Al-Ferrite) showed better corrosion resistance. Its corrosion rate was lower than that of SiC (0.1mm/year), which is a candidate material for the sulfuric acid decomposition reaction vessel. On the other hand, Ni-based alloy pre-filmed with Al$$_{2}$$O$$_{3}$$ is prepared as the relative corrosion film of 3Al-Ferrite. Its corrosion rate was significantly higher than that of 3Al-Ferrite. As the result of EPMA analysis of these oxide films, Ni-based alloy containing 2.4% Si formed Si oxide film which had some cracks after the long term corrosion test. Therefore S penetrated into grain boundaries of the matrix through the oxide film. 3Al-Ferrite formed a thin and uniform Al$$_{2}$$O$$_{3}$$ film, and the penetration of S into the grain boundaries was not observed. Al$$_{2}$$O$$_{3}$$ pre-film of Ni-based alloy also showed S penetration in the matrix because the Al$$_{2}$$O$$_{3}$$ pre-film had many small defects originally. The corrosion oxide film of 3Al-Ferrite consisted of only $$alpha$$-Al$$_{2}$$O$$_{3}$$, while the Al$$_{2}$$O$$_{3}$$ pre-film consist of $$alpha$$-Al$$_{2}$$O$$_{3}$$ and $$gamma$$-Al$$_{2}$$O$$_{3}$$. Those results suggest that the better corrosion resistance of 3Al-Ferrite is due to the uniform formation of dense $$alpha$$-Al$$_{2}$$O$$_{3}$$ film at the early stage of the corrosion.

Journal Articles

Improvement of symbiotic performance of ${it Sinorhizobium}$ with ${it Vigna marina}$ under high salt conditions using the ion beam mutation breeding

Maruyama, Yudai*; Takeda, Kiyoko*; Tomooka, Norihiko*; Sato, Katsuya; Ono, Yutaka; Yokoyama, Tadashi*

JAEA-Review 2015-022, JAEA Takasaki Annual Report 2014, P. 99, 2016/02

https://doi.org/10.11484/jaea-review-2015-022

Journal Articles

Draft genome sequence of ${it Methylobacterium}$ sp. ME121, isolated from soil as a mixed single colony with ${it Kaistia}$ sp. 32K

Fujinami, Shun*; Takeda, Kiyoko*; Onodera, Takefumi*; Sato, Katsuya; Shimizu, Tetsu*; Wakabayashi, Yu*; Narumi, Issey*; Nakamura, Akira*; Ito, Masahiro*

Genome Announcements (Internet), 3(5), p.e01005-15_1 - e01005-15_2, 2015/09

https://doi.org/10.1128/genomeA.01005-15

Journal Articles

Genome analysis of the high temperature tolerant mutant of ${it Bradyrhizobium japonicum}$ USDA110 generated by ion-beam irradiation

Takeda, Kiyoko*; Sato, Katsuya; Narumi, Issey*; Ono, Yutaka; Otsu, Naoko*; Yokoyama, Tadashi*

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 120, 2015/03

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2014-050.pdf#page=152

Journal Articles

Draft genome sequence of calcium-dependent ${it Paenibacillus}$ sp. strain TCA20, isolated from a hot spring containing a high concentration of calcium ions

Fujinami, Shun*; Takeda, Kiyoko*; Onodera, Takefumi*; Sato, Katsuya; Sano, Motohiko*; Takahashi, Yuka*; Narumi, Issey*; Ito, Masahiro*

Genome Announcements (Internet), 2(5), p.e00866-14_1 - e00866-14_2, 2014/09

https://doi.org/10.1128/genomeA.00866-14

Journal Articles

Draft genome sequence of potassium-dependent alkaliphilic ${it Bacillus}$ sp. strain TS-2, isolated from a jumping spider

Fujinami, Shun*; Takeda, Kiyoko*; Onodera, Takefumi*; Sato, Katsuya; Sano, Motohiko*; Narumi, Issey*; Ito, Masahiro*

Genome Announcements (Internet), 2(3), p.e00458-14_1 - e00458-14_2, 2014/05

https://doi.org/10.1128/genomeA.00458-14

Journal Articles

Identification of DNA mutation sites in a high temperature tolerant mutant of ${it Bradyrhizobium japonicum}$ USDA110 generated by ion-beam irradiation

Takeda, Kiyoko; Sato, Katsuya; Narumi, Issey*; Otsu, Naoko*; Yokoyama, Tadashi*

JAEA-Review 2013-059, JAEA Takasaki Annual Report 2012, P. 114, 2014/03

Journal Articles

Mutagenic effect of carbon ion beams in ${it Deinococcus radiodurans}$

Sato, Katsuya; Onodera, Takefumi*; Takeda, Kiyoko; Narumi, Issey*

JAEA-Review 2013-059, JAEA Takasaki Annual Report 2012, P. 110, 2014/03

Journal Articles

Draft genome sequence of sodium-independent alkaliphilic ${it Microbacterium}$ sp. strain TS-1

Fujinami, Shun*; Takeda, Kiyoko; Onodera, Takefumi*; Sato, Katsuya; Sano, Motohiko*; Narumi, Issey*; Ito, Masahiro*

Genome Announcements (Internet), 1(6), P. e01043-13, 2013/12

https://doi.org/10.1128/genomeA.01043-13

Journal Articles

Phenotypic characterization of high temperature tolerant mutants of ${it Bradyrhizobium japonicum}$ USDA110 generated by ion-beam irradiation

Takeda, Kiyoko; Tejima, Kohei*; Sato, Katsuya; Narumi, Issei; Yokoyama, Tadashi*

JAEA-Review 2012-046, JAEA Takasaki Annual Report 2011, P. 107, 2013/01

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2012-046.pdf

Journal Articles

The Effect of $$gamma$$-sterilization of biofertilizer's carrier on bacterial inoculants survival

Tejima, Kohei*; Yokoyama, Tadashi*; Sato, Katsuya; Takeda, Kiyoko; Narumi, Issei

JAEA-Review 2012-046, JAEA Takasaki Annual Report 2011, P. 112, 2013/01

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2012-046.pdf

Journal Articles

The Effect of $$gamma$$-sterilization of carrier materials made with different types of soils on the shelf life of biofertilizer containing ${it Bradyrhizobium japonicum}$ strain USDA110

Tejima, Kohei; Sato, Katsuya; Takeda, Kiyoko; Yokoyama, Tadashi*; Narumi, Issei

Radioisotopes, 61(4), p.161 - 171, 2012/04

https://doi.org/10.3769/radioisotopes.61.161

A biofertilizer is a substance that holds beneficial microorganisms for plant growth in a carrier material. To demonstrate the effect of $$gamma$$-sterilization, the survival of the ${it Bradyrhizobium japonicum}$ was monitored to assess the shelf life of biofertilizers. As biofertilizer carriers, five kinds of typical Japanese soil-based materials were used. Following the sterilization of carrier materials by $$gamma$$-irradiation or autoclaving, ${it B. japonicum}$ was inoculated into each material. The biofertilizer was stored for 12 months at 4$$^{circ}$$C or 30$$^{circ}$$C. After storage, viable inoculants in the biofertilizer were enumerated. Results indicated that inoculant density after storage was greater than the initial density in biofertilizers made from sterilized carriers, whereas it decreased significantly in biofertilizers made from non-sterilized carriers. $$gamma$$-sterilization was superior to autoclave sterilization in enhancing inoculant survival in some cases.

Journal Articles

Colony morphology of high temperature tolerant mutants of ${it Bradyrhizobium japonicum}$ USDA 110 obtained by ion-beam microbial mutation-breeding technology

Takeda, Kiyoko; Tejima, Kohei; Sato, Katsuya; Narumi, Issei; Yokoyama, Tadashi*

JAEA-Review 2011-043, JAEA Takasaki Annual Report 2010, P. 110, 2012/01

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2011-043.pdf

Journal Articles

Phenotypic improvement of ${it Bradyrhizobium japonicum}$ USDA 110 into a high temperature tolerant strain in terms of ion-beam microbial mutation-breeding technology

Takeda, Kiyoko*; Tejima, Kohei; Sato, Katsuya; Narumi, Issei; Yokoyama, Tadashi*

JAEA-Review 2010-065, JAEA Takasaki Annual Report 2009, P. 75, 2011/01

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2010-065.pdf

Oral presentation

Generation of high temperature-tolerant mutants of soybean rhizobium strain USDA110 by ion beam microbial breeding

Yokoyama, Tadashi*; Takeda, Kiyoko*; Tejima, Kohei; Sato, Katsuya; Narumi, Issei

no journal, , 

no abstracts in English

Oral presentation

Generation of high-temperature tolerant mutants of ${it Bradyrhizobium japonicum}$ by ion beam irradiation

Takeda, Kiyoko; Tejima, Kohei; Sato, Katsuya; Narumi, Issei; Yokoyama, Tadashi*

no journal, , 

In Asian countries, many researchers are trying to develop biofertilizers. Major constraint of biofertilizer utilization is a viability loss of beneficial microorganisms caused by high temperature stress during both storage and transportation. Therefore, we have tried to improve high temperature tolerance of soybean nodule bacterium. Consequently, we have obtained 20 high temperature tolerant mutants, which can survive at 43$$^{circ}$$C for 7 days. We also successfully generated 5 mutants, which can survive at 47$$^{circ}$$C for 3 days by re-irradiation to mutants that survive at 43 $$^{circ}$$C for 7 days. On the other hand, we found some phenotypic changes of high temperature tolerant mutants. For example, the colony of these mutants was glossy pale pink color unlike wild type. And the generation time of these mutants at 30$$^{circ}$$C was about 1.5 h shorter than that of wild type. There might be a link between high temperature tolerance and these phenotypic changes.

Oral presentation

Identification of mutation sites in a high temperature tolerant mutant of ${it Bradyrhizobium japonicum}$ USDA110 by ion beam breeding technology

Takeda, Kiyoko; Sato, Katsuya; Narumi, Issey*; Yokoyama, Tadashi*

no journal, , 

We have tried to generate high temperature tolerant mutants of ${it Bradyrhizobium japonicum}$ USDA110 using ion beam breeding technology. Consequently, we obtained a mutant, M14, which can survive at 42$$^{circ}$$C for at least 7 days. The genome sequence data of M14 were accumulated using a GS Junior System. The data were then mapped to the reference sequence of strain USDA110 using a GS Reference Mapper software. About 99.5% of the reference sequence was covered with the M14 sequence. Genome comparison revealed a large-scale structural change in the circular M14 genome. Namely, a DNA region at the coordinates 4,029,225 $$sim$$ 5,299,697 (1,270,473 bp) was inverted. In addition, 84 mutations such as base substitutions, insertions and deletions were found in M14. The acquirement of high temperature tolerance in M14 could be attributed to a large-scale inversion and/or small-scale mutations.

Oral presentation

Genome structure and base mutations in the high temperature tolerant mutant of ${it Bradyrhizobium japonicum}$ USDA110 generated by ion-beam irradiation

Takeda, Kiyoko*; Sato, Katsuya; Ono, Yutaka; Narumi, Issey*; Otsu, Naoko*; Yokoyama, Tadashi*

no journal, , 

no abstracts in English

Oral presentation

Improvement of symbiotic performance of ${it Sinorhizobium}$ with ${it Vigna marina}$ under high salt conditions using the ion beam mutation breeding

Yokoyama, Tadashi*; Maruyama, Yudai*; Takeda, Kiyoko*; Tomooka, Norihiko*; Sato, Katsuya; Ono, Yutaka

no journal, , 

no abstracts in English

Oral presentation

Mutagenic effect of carbon ion beams in ${it Deinococcus radiodurans}$

Sato, Katsuya; Onodera, Takefumi*; Takeda, Kiyoko; Narumi, Issey*

no journal, , 

In this study, we investigated mutant frequencies of two different antibiotic-resistant mutants for carbon ion beams in ${it Deinococcus radiodurans}$ and identified the mutation sites in the streptomycin-resistant (Sm$$^{R}$$) mutants. The Sm$$^{R}$$ mutant frequencies increased depending on radiation dose. It seems that this dose range is the best dose to generate the mutants of interest for research and breeding purpose. Four kinds of mutation sites at the ${it rpsL}$ locus were determined from 8 Sm$$^{R}$$ mutants. These mutations cause amino acid substitutions at position 43 (Lys to Thr or Arg) and 88 (Lys to Glu or Arg) in the S12 protein, respectively. The two hot spots at Lys43 and Lys88 in the S12 protein might be a binding target of streptomycin or adjacent to the center of streptomycin interaction with the ribosome.

33 (Records 1-20 displayed on this page)