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mutant in a salt-tolerant accession revealed an importance of salt acclimation ability in plant salt toleranceAriga, Hirotake*; Katori, Taku*; Yoshihara, Ryohei*; Hase, Yoshihiro; Nozawa, Shigeki; Narumi, Issei; Iuchi, Satoshi*; Kobayashi, Masatomo*; Tezuka, Kenji*; Sakata, Yoichi*; et al.
Plant Signaling & Behavior (Internet), 8(7), p.e24779_1 - e24779_5, 2013/07
Based on analysis of the salinity tolerance among 354 
accessions, some accessions showed greater salt shock tolerance compared with a reference accession, Col-0 on a typical assay with drastic change in NaCl concentration from 0 mM to 225 mM. On the other hand, several accessions including Zu-0 exhibited marked acquired salt tolerance, which is induced after exposure to moderate salt stress (salt acclimation ability). It is likely that Arabidopsis plants have at least two types of tolerance abilities, salt shock tolerance and salt acclimation. To dissect the salt tolerance mechanisms of the salt tolerant accessions, we isolated a salt-sensitive mutant from ion beam-mutagenized Zu-0 seedlings. The mutant showed severe growth inhibition under salt shock stress due to a single base deletion in SOS1 gene as well-known salt shock tolerance gene, even more salt sensitive than Col-0. Nevertheless, the mutant was able to survive on the salt acclimation with 100 mM NaCl for 7 days followed with 750 mM sorbitol for 20 days (salt acclimation assay) as well as the Zu-0 wild type, whereas Col-0 showed apparent chlorosis under the condition. We propose that a gene for salt acclimation ability is different from a gene for salt shock tolerance and plays an important role in acquisition for marked salt- or osmotic tolerance.
-rays and carbon ion beams in 
seedlingsYoshihara, Ryohei*; Nozawa, Shigeki; Hase, Yoshihiro; Narumi, Issei; Hidema, Jun*; Sakamoto, Ayako
Journal of Radiation Research, 54(6), p.1050 - 1056, 2013/05
Times Cited Count：22 Percentile：64.89(Biology)Hase, Yoshihiro; Yoshihara, Ryohei*; Nozawa, Shigeki; Narumi, Issei
JAEA-Review 2012-046, JAEA Takasaki Annual Report 2011, P. 98, 2013/01
Saika, Hiroaki*; Yoshihara, Ryohei*; Sakamoto, Ayako; Toki, Seiichi*
JAEA-Review 2012-046, JAEA Takasaki Annual Report 2011, P. 99, 2013/01
spp.Uyama, Yoshihide*; Oya, Hirotaka*; Amano, Yoshinori*; Kashimoto, Koichi*; Hatano, Shoji*; Nozawa, Shigeki; Yoshihara, Ryohei*; Hase, Yoshihiro; Narumi, Issei
JAEA-Review 2012-046, JAEA Takasaki Annual Report 2011, P. 102, 2013/01
Hase, Yoshihiro; Yoshihara, Ryohei; Nozawa, Shigeki; Narumi, Issei
Mutation Research; Fundamental and Molecular Mechanisms of Mutagenesis, 731(1-2), p.41 - 47, 2012/03
Times Cited Count：33 Percentile：72.04(Biotechnology & Applied Microbiology)To gain insight into the mutagenic effects of accelerated heavy ions in plants, the mutagenic effects of carbon ions near the range end (mean LET: 425 keV/
m) were compared with the effects of carbon ions penetrating the seeds (mean LET: 113 keV/m). Mutational analysis by plasmid rescue of 
from irradiated Arabidopsis plants showed a 2.7-fold increase in mutant frequency for 113 keV/m carbon ions, whereas no enhancement of mutant frequency was observed for carbon ions near the range end. This suggested that carbon ions near the range end induced mutations that were not recovered by plasmid rescue. An Arabidopsis DNA ligase IV mutant, deficient in non-homologous end-joining repair, showed hyper-sensitivity to both types of carbon-ion irradiation. The difference in radiation sensitivity between the wild type and the repair-deficient mutant was greatly diminished for carbon ions near the range end, suggesting that these ions induce irreparable DNA damage. Mutational analysis of the Arabidopsis 
locus showed that while the frequency of generation of glabrous mutant sectors was not different between the two types of carbon-ion irradiation, large deletions (
30 kb) were six times more frequently induced by carbon ions near the range end. This suggests that the proportion of large deletions increases with LET in plants.
Hase, Yoshihiro; Yoshihara, Ryohei; Nozawa, Shigeki; Narumi, Issei
JAEA-Review 2011-043, JAEA Takasaki Annual Report 2010, P. 103, 2012/01
-rays and carbon ion beams on seedlingsYoshihara, Ryohei; Hase, Yoshihiro; Nozawa, Shigeki; Sakamoto, Ayako; Narumi, Issei
JAEA-Review 2010-065, JAEA Takasaki Annual Report 2009, P. 59, 2011/01
Affrida, A. H.*; Zaiton, A.*; Salahbiah, A. M.*; Shakinah, S.*; Yoshihara, Ryohei; Narumi, Issei; Hase, Yoshihiro; Ono, Yutaka
JAEA-Review 2010-065, JAEA Takasaki Annual Report 2009, P. 62, 2011/01
Okada, Tomoyuki*; Ikeda, Hiroshi*; Ono, Yutaka; Yoshihara, Ryohei; Hase, Yoshihiro; Narumi, Issei
JAEA-Review 2010-065, JAEA Takasaki Annual Report 2009, P. 63, 2011/01
no abstracts in English
Matsuo, Yoichi*; Hase, Yoshihiro; Nozawa, Shigeki; Yoshihara, Ryohei; Narumi, Issei
JAEA-Review 2010-065, JAEA Takasaki Annual Report 2009, P. 66, 2011/01
no abstracts in English
Asami, Itsuo*; Tsuji, Takako*; Hasegawa, Toru*; Fukuta, Shiro*; Kuroyanagi, Satoru*; Hase, Yoshihiro; Yoshihara, Ryohei; Narumi, Issei
JAEA-Review 2010-065, JAEA Takasaki Annual Report 2009, P. 68, 2011/01
no abstracts in English
) leaf explantsYotoriyama, Mamoru*; Takano, Junichi*; Namai, Kiyoshi*; Yoshihara, Ryohei; Nozawa, Shigeki; Hase, Yoshihiro; Narumi, Issei
JAEA-Review 2010-065, JAEA Takasaki Annual Report 2009, P. 71, 2011/01
no abstracts in English
Taji, Teruaki*; Horiguchi, Akane*; Ikeda, Akiro*; Nakajima, Chihiro*; Sakata, Yoichi*; Yoshihara, Ryohei; Nozawa, Shigeki; Hase, Yoshihiro; Narumi, Issei
JAEA-Review 2010-065, JAEA Takasaki Annual Report 2009, P. 72, 2011/01
no abstracts in English
Tomikawa, Masaru*; Maeda, Tomoo*; Honda, Kazushige*; Saga, Koichi*; Ishikawa, Ryuji*; Akita, Yusuke; Yoshihara, Ryohei; Nozawa, Shigeki; Narumi, Issei
JAEA-Review 2010-065, JAEA Takasaki Annual Report 2009, P. 73, 2011/01
Yoshihara, Ryohei; Nozawa, Shigeki; Saika, Hiroaki*; Teranishi, Mika*; Toki, Seiichi*; Hidema, Jun*; Sakamoto, Ayako
JAEA-Review 2010-065, JAEA Takasaki Annual Report 2009, P. 74, 2011/01
transgenic Yoshihara, Ryohei; Hase, Yoshihiro; Sato, Ryohei*; Takimoto, Koichi*; Narumi, Issei
International Journal of Radiation Biology, 86(2), p.125 - 131, 2010/02
Times Cited Count：21 Percentile：80.21(Biology)In an effort to assess the characteristics of mutation induced by different LET radiation in higher plants, the mutational effects of carbon-ion beams and -rays were investigated in . The transgenic (Arabidopsis/rpsL) mutation detection system was adopted. Dry seeds of Arabidopsis/rpsL were irradiated with -rays and 208-MeV carbon ions (208-MeV 
C
), and the mutation frequency and mutation spectrum were examined. Our result suggested that 208-MeV C and -rays induced different mutational effects in dry seed.
-rays and carbon ion beams in plantYoshihara, Ryohei; Hase, Yoshihiro; Nozawa, Shigeki; Narumi, Issei
JAEA-Review 2009-041, JAEA Takasaki Annual Report 2008, P. 67, 2009/12
Affrida, A. H.*; Shakinah, S.*; Zaiton, A.*; Yoshihara, Ryohei; Narumi, Issei; Hase, Yoshihiro; Ono, Yutaka
JAEA-Review 2009-041, JAEA Takasaki Annual Report 2008, P. 70, 2009/12
C
beam irradiated-lateral meristems and -isolated cultured cellsTsuro, Masato*; Inazawa, Marie*; Nozawa, Shigeki; Yoshihara, Ryohei; Hase, Yoshihiro; Narumi, Issei
JAEA-Review 2009-041, JAEA Takasaki Annual Report 2008, P. 71, 2009/12
no abstracts in English
