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論文

Comparative effects of radon inhalation according to mouse strain and cisplatin dose in a cisplatin-induced renal damage model

笹岡 香織*; 片岡 隆浩*; 神崎 訓枝; 小橋 佑介*; 迫田 晃弘; 石森 有; 山岡 聖典*

Pakistan Journal of Zoology, 50(3), p.1157 - 1170, 2018/06

 パーセンタイル:100(Zoology)

シスプラチン(CDDP)は固形がんの治療に広く使用されているが、酸化ストレスによる腎毒性を持っている。そこで、我々は、ラドン吸入による放射線感受性の違いによるマウス系統差によるCDDP誘導腎障害に対する影響とCDDP濃度について検討した。まず、C57BL/6JまたはBALB/cのマウスに、1000Bq/m$$^{3}$$または2000Bq/m$$^{3}$$のラドンを24時間吸入後、CDDPを20mg/kg体重投与した。C57BL/6Jでは毛並等が改善され、BALB/cでは悪化した。次に、BALB/cマウスに、1000Bq/m$$^{3}$$のラドンを24時間後、CDDPを15mg/kg体重投与した。その結果、抗酸化機能の亢進などが見られた。以上のことから、ラドン吸入によってCDDP誘導腎障害を抑制することが可能であることが示唆された。

口頭

Oxidative stress suppresses locomotion and pumping motion in $textit{Caenorhabditis elegans}$

鈴木 芳代; 坂下 哲哉; 服部 佑哉; 小林 泰彦

no journal, , 

We recently found that whole body irradiation reduced locomotion in $textit{C. elegans}$, though the mechanisms are not clear. It is well known that free radicals such as OH$$^-$$ and H$$^-$$ are produced following exposure of ionizing radiation. The reactions of free radicals cause the production of oxidative stress including hydrogen peroxide (H$$_2$$O$$_2$$). Oxidative stress is known as an important factor of aging, and may relate to the decrease of motility observed in the aging process. We therefore explored the potential effectiveness of H$$_2$$O$$_2$$ in the suppression of $textit{C. elegans}$'s locomotion. Wild-type animals were exposed to graded doses of H$$_2$$O$$_2$$ and the locomotory rate was evaluated. The locomotory rate was significantly decreased in H$$_2$$O$$_2$$-exposed animals, in which the dose response was similar to that in the irradiated animals. We subsequently investigated the radiation effects on the pharyngeal pumping motion (chewing and swallowing) and the involvement of oxidative stress. As a result, the proportion of the pumping-motion arrest significantly increased after whole body irradiation and the pumping-motion arrest was restored within several hours. Furthermore, the response in H$$_2$$O$$_2$$-exposed animals was similar to that in the irradiated animals. These results support the possibility that radiation-induced suppression of both locomotion and pumping motion in $textit{C. elegans}$ was caused by radiation-produced H$$_2$$O$$_2$$.

口頭

Ionizing radiation and oxidative stress suppress locomotion and pharyngeal pumping motion in ${it Caenorhabditis elegans}$

鈴木 芳代; 坂下 哲哉; 服部 佑哉; 小林 泰彦

no journal, , 

Oxidative stress such as hydrogen peroxide (H$$_2$$O$$_2$$) has been identified as one of the important factors in aging, and this may be related to the decrease in motility observed during the aging process. In the present study, we therefore explored the potential effectiveness of H$$_2$$O$$_2$$ in the suppression of ${it C. elegans}$' locomotion (crawling). Young adult wild-type animals were exposed to S-basal buffer containing graded doses of H$$_2$$O$$_2$$. Exposure to H$$_2$$O$$_2$$ induced reduction of locomotion similar to that observed after whole-body irradiation. To investigate the radiation effects and the involvement of oxidative stress on other types of movement, we subsequently focused on the pharyngeal pumping motion (chewing and swallowing). The proportion of animals in which the pumping motion has stopped immediately after H$$_2$$O$$_2$$ exposure increased. This response was similar to that of irradiated animals. These results support the possibility that radiation-induced suppression of both locomotion and pumping motion in ${it C. elegans}$ was caused by radiation-produced H$$_2$$O$$_2$$. In this presentation, we will give an outline of the radiation effects on ${it C. elegans}$ and discuss the involvement of the radiation-induced oxidative stress in the phenomena.

口頭

Detection and measurement of abnormal posture under stressful conditions in age-related mutants

Matsuda, Sakiyo*; Nitta, Nazuki*; Tamura, Miku*; 鈴木 芳代; 坂下 哲哉; 石井 直明*; 簗瀬 澄乃*

no journal, , 

Oxidative stress threatens cellular constructs including lipids, proteins and nucleic acids. As a result, oxidative stress causes or influence apoptosis, degenerative diseases and aging. Recently, we found an abnormal posture in short-lived mutants such as $$mev-1$$ and $$daf-16$$, exposed to hyperoxia. In this study, we detected the hyperoxia-induced abnormal posture in $$mev-1$$ and $$daf-16$$ mutants compared with wild-type N2 and long-lived $$age-1$$ strains. 4-day-old animals placed on a NGM agar plate with a fixed bacterial lawn were exposed to hyperoxia (90% of oxygen) overnight. After the exposure, some animals were immediately recorded as digital images with up to 400-fold magnification of the microscope. The images were analyzed using image-processing software by a previously published method. To evaluate the posture of the body, we introduced a novel standard, namely the "posture index" as the absolute average of the relative angle between adjacent dividing points. As a result of this investigation, we can not only intuitively but also quantitatively and statistically understand a tendency of the behavior in various postural responses under stressful conditions in the short-lived mutants.

口頭

ラドン吸入によるマウス諸臓器中の過酸化水素産生に関する基礎的検討

片岡 隆浩*; 神崎 訓枝; 迫田 晃弘; 石田 毅; 首藤 妃奈*; 矢野 準喜*; 田中 裕史; 花元 克巳*; 寺東 宏明*; 光延 文裕*; et al.

no journal, , 

これまでラドン吸入によってマウス臓器中の抗酸化機能が亢進することを報告してきた。ラドン吸入による抗酸化機能の亢進のメカニズムとして、微量のROSの生成が関わっている可能性が高い。そこで、本発表では、ラドンを吸入したマウス諸臓器中の抗酸化機能の活性化と過酸化水素量について調べたので報告する。

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