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Development of high-throughput analysis system using highly-functional organic polymer monoliths

Umemura, Tomonari*; Kojima, Norihisa*; Ueki, Yuji

The growing demand for high-throughput analysis in the current competitive life sciences and industries has promoted the development of high-speed HPLC techniques and tools. As one of such tools, monolithic columns have attracted increasing attention and interest in the last decade due to the low flow-resistance and excellent mass transfer, allowing for rapid separations and reactions at high flow rates with minimal loss of column efficiency. In the present paper, recent progress in the development of organic polymer monoliths is discussed. Especially, the procedure for the preparation of methacrylate-based monoliths with various functional groups is described. Furthermore, the performance of the produced monoliths is demonstrated through the results for (1) rapid separations of alkylbenzenes at high flow rates, (2) flow-through enzymatic digestion of cytochrome c on a trypsin-immobilized monolithic column, and (3) separation of the tryptic digest on a reversed-phase monolithic column.

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Category:Chemistry, Analytical

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