PET imaging and biodistribution of Cu labeled monoclonal antibody

Cu標識モノクローナル抗体のPETイメージングと体内分布

Paudyal, P.*; Paudyal, B.*; 飯田 靖彦*; 花岡 宏史*; 渡辺 智; 石岡 典子; 渡邉 茂樹; 高柳 恵美*; 吉岡 弘樹*; 織内 昇*; 富永 英之*; 樋口 徹也*; 遠藤 啓吾*

Paudyal, P.*; Paudyal, B.*; Iida, Yasuhiko*; Hanaoka, Hirofumi*; Watanabe, Satoshi; Ishioka, Noriko; Watanabe, Shigeki; Takayanagi, Megumi*; Yoshioka, Hiroki*; Oriuchi, Noboru*; Tominaga, Hideyuki*; Higuchi, Tetsuya*; Endo, Keigo*

Quantitative PET imaging using Cu labeled antibody was investigated by animal study. Anti CD20 antibody, NuB2 was conjugated with the chelator, DOTA and radiolabeled with Cu. For animal study, nude mice bearing lymphoma (Raji tumor) were injected with Cu-DOTA-NuB2. The PET imaging followed by the biodistribution studies were done at 10min, 24h and 48h post injection. PET imaging showed the significant Cu-DOTA-NuB2 accumulations in the tumor. Biodistribution studies also revealed the significant uptake in tumor than that in muscle region at any time point. This study demonstrated the utility of Cu labeled antibody as a diagnostic imaging agent for PET and the success can be translated into clinic for the quantitative targeted imaging with PET.