PET imaging and biodistribution of
Cu labeled monoclonal antibody
Cu標識モノクローナル抗体のPETイメージングと体内分布
Paudyal, P.*; Paudyal, B.*; 飯田 靖彦*; 花岡 宏史*; 渡辺 智; 石岡 典子; 渡邉 茂樹; 高柳 恵美*; 吉岡 弘樹*; 織内 昇*; 富永 英之*; 樋口 徹也*; 遠藤 啓吾*
Paudyal, P.*; Paudyal, B.*; Iida, Yasuhiko*; Hanaoka, Hirofumi*; Watanabe, Satoshi; Ishioka, Noriko; Watanabe, Shigeki; Takayanagi, Megumi*; Yoshioka, Hiroki*; Oriuchi, Noboru*; Tominaga, Hideyuki*; Higuchi, Tetsuya*; Endo, Keigo*
Quantitative PET imaging using
Cu labeled antibody was investigated by animal study. Anti CD20 antibody, NuB2 was conjugated with the chelator, DOTA and radiolabeled with
Cu. For animal study, nude mice bearing lymphoma (Raji tumor) were injected with
Cu-DOTA-NuB2. The PET imaging followed by the biodistribution studies were done at 10min, 24h and 48h post injection. PET imaging showed the significant
Cu-DOTA-NuB2 accumulations in the tumor. Biodistribution studies also revealed the significant uptake in tumor than that in muscle region at any time point. This study demonstrated the utility of
Cu labeled antibody as a diagnostic imaging agent for PET and the success can be translated into clinic for the quantitative targeted imaging with PET.