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Report No.

Ion-beam and $$gamma$$-ray irradiations induce thermotolerant mutants in the entomopathogenic fungus ${it Metarhizium anisopliae s.l.}$

Fitriana, Y.*; Sato, Katsuya; Narumi, Issey*; Saito, Tsutomu*

Entomopathogenic fungi, such as ${it Metarhizium anisopliae}$ Sorokin, are important agents for the biological control of insect pests. However, these fungi are not compatible with high temperatures. In this study, mutagenesis using ion beams or $$gamma$$ rays was used to generate five potentially thermotolerant mutants from two wild-type isolates of ${it M. anisopliae}$ (two using ion beams and three using $$gamma$$ rays). The mutant isolates had a higher upper thermal limit for vegetative growth compared to the wild types (by 2-3$$^{circ}$$C) and enhanced tolerance to wet-heat stress of 45$$^{circ}$$C for conidial germination. These results indicate that ion beams and $$gamma$$ rays are useful tools for improving biological characteristics, such as thermotolerance, in entomopathogenic fungi, but that mutants must be carefully evaluated for unpredictable negative side effects.



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Category:Biotechnology & Applied Microbiology



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