Disruption analysis of the gene in

における遺伝子の破壊解析

鳴海 一成*; 小森谷 甲*; 佐藤 勝也

Narumi, Issey*; Komoriya, Kan*; Sato, Katsuya

Other groups failed to generate the disruptant strains, suggesting an essential role of in . is an extremely radiation resistant bacterium closely related to . We attempted to generate a disruptant strain of to determine whether the gene is essential for cell viability in . As a result, we could generate a complete disruptant strain. Although the disruptant strain exhibited slightly reduced growth relative to the wild-type strain, the sensitivity of the disruptant strain to UV-C is comparable to that of the wild-type strain, suggesting a very minor role of RecJ for repair of DNA damage induced by UV-C in . On the other hand, the loss of caused a drastic morphological change in cells. The filamentation phenotype suggests an important role of RecJ in normal cell division of .