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Tanaka, Masaru*; Kawara, Osami*; Ishizaka, Kaoru*; Ohata, Yuki*; Fukuike, Iori*; Kawase, Keiichi; Tokizawa, Takayuki; Miyagawa, Hiroshi*; Ishimori, Yuu
JAEA-Research 2018-001, 98 Pages, 2018/06
JAEA-Research-2018-001.pdf:2.49MB
In the 2016 fiscal year, communication cases on general waste disposal facility construction plans in recent years were surveyed. Results suggested as follows: (1) Existing long-term relationships or agreements in local area promote local accepting. (2) An operator needs to consider alternative plans and explain reasons for the decision making to local stakeholders. (3) Even after first announcement of a new plan, an operator needs to review the plan depending on local concerns. (4) Announcement of a new plan will activate communications on local development including the site redevelopment.
Tanaka, Masaru*; Aoyama, Isao*; Ishizaka, Kaoru*; Ohata, Yuki*; Fukuike, Iori*; Kawase, Keiichi; Watanabe, Masanori; Tokizawa, Takayuki; Miyagawa, Hiroshi*; Ishimori, Yuu
JAEA-Research 2017-003, 65 Pages, 2017/06
JAEA-Research-2017-003.pdf:2.92MB
JAEA Ningyo-toge Environmental Engineering Center and Fukushima Environmental Safety Center have same challenges in risk communication. As reference, similar domestic cases were investigated by our two Centers, and requirements for building long-term relationship were clarified. As follows; (1) Develop new relationship with various stakeholders in the region. (2) Make better use of existing resources (personnel, land and facilities, etc.). (3) Make a concerted effort to create new values with local stakeholders. (4) Make an opportunity which local stakeholders confirm safety and build confidence to the project. These efforts will enhance the opportunities for operators and residents to learn about environment management and environmental protection.
Tanaka, Masaru*; Aoyama, Isao*; Ishizaka, Kaoru*; Ohata, Yuki*; Fukuike, Iori*; Miyagawa, Hiroshi*; Ishimori, Yuu
JAEA-Research 2016-017, 76 Pages, 2017/01
JAEA-Research-2016-017.pdf:10.57MB
From 1955 to 2001, Japan Atomic Energy Agency (JAEA) carried out research and development projects related to uranium exploration, mining refining, conversion and enrichment at/around Ningyo-toge in Japan. Subsequently, JAEA has been conducting projects related to decommissioning of nuclear fuel cycle facilities and remediation of closed mine sites. JAEA had opportunities of communication with local stakeholders through the projects. Consensus building with local stakeholders and maintain it for over decades are important challenges for JAEA Ningyo-toge Environmental Engineering Center. For this aim, similar domestic case were investigated and compared, and evaluated about required measures for long term relationship with local stakeholder.
-transferase gene in cyclamenKitamura, Satoshi; Akita, Yusuke; Ishizaka, Hiroshi*; Narumi, Issei; Tanaka, Atsushi
Journal of Plant Physiology, 169(6), p.636 - 642, 2012/04
Times Cited Count：85 Percentile：91.26(Plant Sciences)In order to identify the anthocyanin-related GST in cyclamen, four candidates of GSTs (CkmGST1 to CkmGST4) were isolated. Phylogenetic analysis indicated that CkmGST3 was closely related to PhAN9, an anthocyanin-related GST of petunia. Expression analysis at different developmental stages of petals revealed that CkmGST3 was strongly expressed in paler pigmented petals than in fully pigmented petals, in contrast to the constitutive expression of the other three candidates during petal development. This expression pattern of CkmGST3 was correlated with those of other anthocyaninbiosynthetic genes such as CkmF3'5'H and CkmDFR2. Molecular complementation of Arabidopsis 
, a knockout mutant of an anthocyanin-related GST gene, demonstrated that CkmGST3 could complement the anthocyanin-less phenotype of . Transgenic plants that expressed the other three CkmGSTs did not show anthocyanin accumulation. These results indicate CkmGST3 functions in anthocyanin accumulation in cyclamen.
-methyltransferase involved in flower colorationAkita, Yusuke; Kitamura, Satoshi; Hase, Yoshihiro; Narumi, Issei; Ishizaka, Hiroshi*; Kondo, Emiko*; Kameari, Naoko*; Nakayama, Masayoshi*; Tanikawa, Natsu*; Morita, Yasumasa*; et al.
Planta, 234(6), p.1127 - 1136, 2011/12
Times Cited Count：44 Percentile：75.48(Plant Sciences)
spp., generated by ion-beam irraidationKondo, Emiko*; Nakayama, Masayoshi*; Kameari, Naoko*; Tanikawa, Natsu*; Morita, Yasumasa*; Akita, Yusuke; Hase, Yoshihiro; Tanaka, Atsushi; Ishizaka, Hiroshi*
JAEA-Review 2010-065, JAEA Takasaki Annual Report 2009, P. 65, 2011/01
no abstracts in English
Akita, Yusuke; Ishizaka, Hiroshi*; Nakayama, Masayoshi*; Shimada, Akihiko; Kitamura, Satoshi; Hase, Yoshihiro; Narumi, Issei; Tanaka, Atsushi
Journal of Horticultural Science & Biotechnology, 85(5), p.437 - 443, 2010/09
The flower colour of gra6 (wild-type) is pink-purple in the flower. On the other hand, flowers of 
gra50 (white-flowered variant) exhibit a white colour in its flower. In this study, the relationship between floral pigmentation and expression of several anthocyanin biosynthesis genes was investigated in gra6 and gra50. The pigments in the flower consist mainly of malvidin 3,5-diglucoside in gra6. White-flowered gra50 possessed lower amounts of anthocyanins, but higher amounts of flavonols compared to gra6. Gene expression analysis demonstrated that expression of the dihydroflavonol 4-reductase gene 2 (
) was lower in gra50 compared with gra6, whereas the expression of other key genes did not differ greatly. These results suggest that the white-flowered variant of gra50 may result from a defect in expression of the gene.
spp., generated by ion-beam irradiationKondo, Emiko*; Nakayama, Masayoshi*; Kameari, Naoko*; Tanikawa, Natsu*; Morita, Yasumasa*; Akita, Yusuke; Hase, Yoshihiro; Tanaka, Atsushi; Ishizaka, Hiroshi*
Plant Biotechnology, 26(5), p.565 - 569, 2009/01
Times Cited Count：37 Percentile：68.06(Biotechnology & Applied Microbiology)Fragrant cyclamen cultivar (
), that bloomed purple flower containing malvidin 3,5-diglucoside as the major anthocyanin, was irradiated with a 320-MeV carbon-ion beam at 0-16 Gy to increase flower color variation by mutation. Some of the M2 plants derived from self-pollination of M1 plants irradiated at 2 Gy were flower-color mutants that retained desirable flower shape, flower size, and leaf color. One of the mutants bloomed novel red-purple flowers, the major anthocyanin of which was delphinidin 3,5-diglucoside. Because the major anthocyanins in flowers of spp. were previously restricted to malvidin, peonidin, and cyanidin types, the generation of a cyclamen containing mostly the delphinidin-type anthocyanin is an important breakthrough in cyclamen breeding. We expect this mutant to become not only a commercial cultivar itself, but also a valuable genetic resource for cyclamen breeding.
)Kondo, Emiko*; Hase, Yoshihiro; Narumi, Issei; Ishizaka, Hiroshi*
JAEA-Review 2008-055, JAEA Takasaki Annual Report 2007, P. 79, 2008/11
no abstracts in English
Ishida, Toshihisa; Imayoshi, Sho*; Yoritsune, Tsutomu; Nunokaya, Hiroshi*; Ochiai, Masaaki; Ishizaka, Yuichi*
Journal of Nuclear Science and Technology, 38(7), p.557 - 570, 2001/07
Times Cited Count：21 Percentile：79.60(Nuclear Science & Technology)no abstracts in English
)Kondo, Emiko*; Hase, Yoshihiro; Tanaka, Atsushi; Ishizaka, Hiroshi*
no journal, ,
no abstracts in English
Kondo, Emiko*; Ishizaka, Hiroshi*; Hase, Yoshihiro; Narumi, Issei
no journal, ,
no abstracts in English
Kondo, Emiko*; Nakayama, Masayoshi*; Kameari, Naoko*; Tanikawa, Natsu*; Morita, Yasumasa*; Akita, Yusuke; Hase, Yoshihiro; Tanaka, Atsushi; Ishizaka, Hiroshi*
no journal, ,
One of the fragrant cyclamens, Kaori-no-mai, blooms purple flowers containing malvidin 3,5-diglucoside as the major anthocyanin. Here, we irradiated etiolated petioles of Kaori-no-mai with a 320-MeV carbon-ion beam to increase flower color variation by mutation. Some of the M2 plants derived from self-pollination of M1 plants were flower-color mutan. One of the mutants bloomed novel red-purple flowers, the major anthocyanin of which was delphinidin 3,5-diglucoside. The major anthocyanins in flowers of 
spp. were previously restricted to malvidin, peonidin, and cyanidin types, therefore, the generation of a cyclamen containing mostly the delphinidin-type anthocyanin is an important breakthrough in cyclamen breeding. We expect this mutant to become not only a commercial cultivar itself, but also a valuable genetic resource for cyclamen breeding.
Kondo, Emiko*; Kameari, Naoko*; Nakayama, Masayoshi*; Kurihara, Yasushi*; Akita, Yusuke; Tanikawa, Natsu*; Morita, Yasumasa*; Hase, Yoshihiro; Tanaka, Atsushi; Ishizaka, Hiroshi*
no journal, ,
no abstracts in English
Kondo, Emiko*; Kameari, Naoko*; Ishizaka, Hiroshi*; Nakayama, Masayoshi*; Tanikawa, Natsu*; Morita, Yasumasa*; Akita, Yusuke; Hase, Yoshihiro; Tanaka, Atsushi
no journal, ,
no abstracts in English
Kitamura, Akane; Yuri, Yosuke; Yuyama, Takahiro; Ishizaka, Tomohisa; Yamaki, Tetsuya; Koshikawa, Hiroshi; Sawada, Shinichi
no journal, ,
no abstracts in English
"Golden Boy" ) by ion-beam irradiationKameari, Naoko*; Akita, Yusuke; Kitamura, Satoshi; Hase, Yoshihiro; Kondo, Emiko*; Nakayama, Masayoshi*; Kurihara, Yasushi*; Tanikawa, Natsu*; Morita, Yasumasa*; Tanaka, Atsushi; et al.
no journal, ,
no abstracts in English
Akita, Yusuke; Kitamura, Satoshi; Hase, Yoshihiro; Narumi, Issei; Ishizaka, Hiroshi*; Kondo, Emiko*; Kameari, Naoko*; Nakayama, Masayoshi*; Tanikawa, Natsu*; Morita, Yasumasa*; et al.
no journal, ,
no abstracts in English
Akita, Yusuke*; Kitamura, Satoshi; Hase, Yoshihiro; Narumi, Issey*; Ishizaka, Hiroshi*; Kondo, Emiko*; Kameari, Naoko*; Nakayama, Masayoshi*; Tanaka, Atsushi
no journal, ,
In this study, using several flower-colored mutants and their parents, we analyzed flavonoid composition in flowers by HPLC, and isolated anthocyanin biosynthetic genes by degenerate PCR. We isolated several anthocyanin biosynthetic genes from C. graecum, C. purpurascens and fragrant cyclamen cultivars. Then, we analyzed their expression patterns between wild-type and flower-colored mutants. Based on their comparison, we selected candidates for anthocyanin biosynthetic genes and confirmed their enzymatic activities in vitro. Furthermore, we also demonstrated the effectiveness of PCR-based screening method in early growth stage of mutagenized cyclamen, using newly developed DNA markers.
) by ion-beam irradiation and analysis of their flower pigmentsKondo, Emiko*; Nakayama, Masayoshi*; Kameari, Naoko*; Tanikawa, Natsu*; Morita, Yasumasa*; Kitamura, Satoshi; Akita, Yusuke; Hase, Yoshihiro; Tanaka, Atsushi; Ishizaka, Hiroshi*
no journal, ,
no abstracts in English
